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090			_aB-19686
245	1	0	_aChemometrics-enhanced high-performance liquid chromatography-diode array detection strategy to quantify protob erb erine alkaloids in varying Coptidis Rhizoma-related medicines
490	0		_vJournal of Chromatography A, 1681, p.463426, 2022
520	3		_aQuantitation of protoberberine alkaloids is an essential guarantee for efficacy control and medication safety of Coptidis Rhizoma (CR)related medicines. Traditional univariate chromatography faced challenges with co-elution, unknown interferences, and retention time shift when analyzing isomeric analytes in varying sample matrices. We presented a chemometrics-enhanced high-performance liquid chromatography-diode array detection (HPLC-DAD)strategy for simultaneous quantification of six protoberberine alkaloids and processed multi-channels chromatographic-spectral data with four second-order calibration algorithms. Chromatographic conditions were firstly optimized. Four groups of predicted samples were modeled individually with the designed calibration set. Mathematical resolutions were then obtained, and pseudo-univariate regression gave the quantitative concentration of each analyte. Four models were scored on fit, linearity, recovery, and robustness, where alternating trilinear decomposition assisted multivariate curve resolution (ATLD-MCR)exhibited an optimal and stable performance. Besides, the resolved spectra presented high consistency with the actual spectra (r?0.9993). Limits of quantification (LOQ)fully met the pharmacopoeia stipulation and were 0.17, 0.60, 0.19, 0.74, 0.15, and 0.38 µg mL-1 for columbamine, epiberberine, jatrorrhizine, coptisine, palmatine, and berberine, respectively. The importance of this strategy is to exploit collinearity resolution and additional selectivity that permit accurate quantitation at poor chromatographic resolutions, avoiding individual pretreatment and HPLC optimizations for different samples. This study provides a universal alternative for routine quality assessment of protoberberine alkaloids in CR-related medicines.
650	1	4	_aCOPTIDIS RHIZOMA
650	1	4	_aHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
650	1	4	_aMATHEMATICAL SEPARATION
650	1	4	_aPROTOBERBERINE ALKALOIDS
650	1	4	_aSECOND-ORDER CALIBRATION
700	1	2	_aChen, A. Q.
700	1	2	_aWu, H. L.
700	1	2	_aSun, H. B.
700	1	2	_aWang, X. Z.
700	1	2	_aYan, X. Q.
700	1	2	_aWang, T.
700	1	2	_aYu, R. Q.
856	4	0	_uhttps://drive.google.com/file/d/1Oj27wZqO1JdwByzLN1jzfUeNpNw_QPaS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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