| 000 | 02485nam a2200289Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625162436.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-19727 | ||
| 245 | 1 | 0 | _aStress responsiveness of vindoline accumulation in Catharanthus roseus leaves is mediated through co.expression of allene oxide cyclase with pathway genes |
| 490 | 0 | _vProtoplasma, 259(3), p.755-773, 2022 | |
| 520 | 3 | _aVindoline is an important alkaloid produced in Catharanthus roseus leaves. It is the more important monomer of the scarce and costly anticancer bisindole alkaloids, vincristine, and vinblastine, as unlike catharanthine (the other monomer), its biosynthesis is restricted to the leaves. Here, biotic (bacterial endophyte, phytoplasma, virus)and abiotic (temperature, salinity, SA, MeJa)factors were studied for their effect on vindoline accumulation in C. roseus. Variations in vindoline pathway-related gene expression were reflected in changes in vindoline content. Since allene oxide cyclase (CrAOC)is involved in jasmonate biosynthesis and MeJa modulates many vindoline pathway genes, the correlation between CrAOC expression and vindoline content was studied. It was taken up for full-length cloning, tissue-specific expression profiling, in silico analyses, and upstream genomic region analysis for cis-regulatory elements. Co-expression analysis of CrAOC with vindoline metabolism-related genes under the influence of aforementioned abiotic/biotic factors indicated its stronger direct correlation with the tabersonine-to-vindoline genes (t16h, omt, t3o, t3r, nmt, d4h, dat)as compared to the pre-tabersonine genes (tdc, str, sgd). Its expression was inversely related to that of downstream-acting peroxidase (prx)(except under temperature stress). Direct/positive relationship of CrAOC expression with vindoline content established it as a key gene modulating vindoline accumulation in C. roseus. | |
| 650 | 1 | 4 | _aABIOTIC/BIOTIC FACTORS |
| 650 | 1 | 4 | _aBISINDOLE ALKALOIDS |
| 650 | 1 | 4 | _aCO-EXPRESSION ANALYSIS |
| 650 | 1 | 4 | _aJASMONATE |
| 650 | 1 | 4 | _aVINDOLINE METABOLISM |
| 700 | 1 | 2 | _aMall, M. |
| 700 | 1 | 2 | _aShanker, K. |
| 700 | 1 | 2 | _aSamad, A. |
| 700 | 1 | 2 | _aKalra, A. |
| 700 | 1 | 2 | _aSundaresan, V. |
| 700 | 1 | 2 | _aShukla, A. K. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1DzdTaRhyeSNZOURdkDXpcaoUKws9v_NZ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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