| 000 | 02276nam a2200313Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162437.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-19823 | ||
| 245 | 1 | 0 | _aThe S40 family members delay leaf senescence by promoting cytokinin synthesis |
| 490 | 0 | _vPlant Physiology and BioChemistry, 191, p.99-109, 2022 | |
| 520 | 3 | _aLeaf senescence is regulated by both endogenous hormones and environmental stimuli in a programmed and concerted way. The members of the S40 family have been reported to play roles in leaf senescence. Here we identified an S40 family member, CiS40-11, from Caragana intermedia. Phylogenetic analysis revealed that the CiS40-11 protein had the highest identity with AtS40-5 (AT1G11700)and AtS40-6 (AT1G61930)of Arabidopsis thaliana. CiS40-11 was highly expressed in leaves and was down-regulated after dark treatment. The subcellular localization analysis showed that CiS40-11 was a cytoplasm-nucleus dual-localized protein. Leaf senescence was delayed in both the CiS40-11 overexpressed A. thaliana and its transiently expressed C. intermedia. Transcriptomic analysis and endogenous hormones assay revealed that CiS40-11 inhibited leaf senescence via promoting the biosynthesis of cytokinins by blocking AtMYB2 expression in the CiS40-11 overexpression lines. Furthermore, overexpression of either AtS40-5 or AtS40-6 showed similar phenotype as the CiS40-11 overexpressing lines, while in the ats40-5a or ats40-6a mutants, the AtMYB2 expression was increased and their leaves exhibited a premature senescence phenotype. These results provide a new molecular mechanism of the S40 family in leaf senescence regulation of plants. | |
| 650 | 1 | 4 | _aARABIDOPSIS THALIANA |
| 650 | 1 | 4 | _aATMYB2 |
| 650 | 1 | 4 | _aCARAGANA INTERMEDIA |
| 650 | 1 | 4 | _aCYTOKININS |
| 650 | 1 | 4 | _aLEAF SENESCENCE |
| 650 | 1 | 4 | _aSENESCENCE REGULATOR (S40) |
| 700 | 1 | 2 | _aYang, T. |
| 700 | 1 | 2 | _aZhang, M. |
| 700 | 1 | 2 | _aYang, Q. |
| 700 | 1 | 2 | _aLiu, K. |
| 700 | 1 | 2 | _aCui, J. |
| 700 | 1 | 2 | _aChen, J. |
| 700 | 1 | 2 | _aLi, G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1Bwkkm4mjsmODo0-6np7CK80NohVfpm-K/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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