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090			_aB-19901
245	1	0	_aAntifungal activity and molecular mechanisms of mulberrin derivatives against Colletotrichum gloeosporioides for mango storage
490	0		_vInternational Journal of Food MicroBiology, 378, p.109817, 2022
520	3		_aIn this work, by using high throughput virtual screening and bioactivity assays, this work revealed that three natural compounds, mulberrin (Mul)exhibiting the highest anti-CYP51 activity, isoxanthohumol and (s)-isopsoralen markedly inhibited 14?-demethylase (a pivotal biosynthetic enzyme involved in the biosynthesis of ergosterol)in Colletotrichum gloeosporioides. Results of computational biology analysis demonstrated that, among the three inhibitors bound to the catalytic pocket of CYP51, Mul showed a closer distance with heme in CYP51 and a stronger binding free energy with CYP51. In vitro tests, Mul demonstrated excellent anti-Colletotrichum gloeosporioides activity by inhibiting CYP51 activity. Notably, Mul treatment decreased the bioactivity of CYP51, thereby increasing cell membrane permeability and cell death. Moreover, Mul treatment significantly prolonged the preservation period of fruits. These results suggest that Mul suppresses anthracnose in postharvest mango by inhibiting the growth of Colletotrichum gloeosporioides and can be used as a potential natural preserving agent.
650	1	4	_a14?-DEMETHYLASE
650	1	4	_aFRUIT PRESERVATIVES
650	1	4	_aINHIBITORS
650	1	4	_aMOLECULAR MODELING
700	1	2	_aNiu, X.
700	1	2	_aLin, L.
700	1	2	_aLiu, L.
700	1	2	_aYu, Y.
700	1	2	_aWang, H.
856	4	0	_uhttps://drive.google.com/file/d/1sHmeU2OADujDyRSgfO0MAZazCqAJMwgS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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