| 000 | 02341nam a2200301Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162440.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20002 | ||
| 245 | 1 | 0 | _aGenome-wide identification of glutamate synthase gene family and expression patterns analysis in response to carbon and nitrogen treatment in Populus |
| 490 | 0 | _vGene, 851, p.146996, 2023 | |
| 520 | 3 | _aGlutamate synthase (GOGAT)is a key enzyme in glutamine synthetase (GS)/GOGAT cycle and at the hub of carbon and nitrogen metabolism, catalyzing the formation of glutamate from ?-oxoglutarate and glutamine. In this study, members of GOGAT family in Populus trichocarpa were identified and analyzed by bioinformatics. The four PtGOGATs were divided into two subgroups: subgroup A (Fd-GOGAT1 and Fd-GOGAT2)and subgroup B (NADH-GOGAT1 and NADH-GOGAT2). Many important elements have been identified in the promoters of different PtGOGATs, including hormone- and light-responsive elements. Meanwhile, the transcript levels of PxGOGATs were affected by light and diurnal cycle. Quantitative real-time PCR showed PxFd-GOGATs and PxNADH-GOGATs were mainly expressed in leaves and roots in Populus × xiaohei T. S. Hwang et Liang, respectively. Under elevated CO2, PxGOGATs were suppressed in all tissues except the stem. And PxFd-GOGATs and PxNADH-GOGATs were strongly induced by nitrogen in leaves and roots, respectively. In addition, PxGOGATs were stimulated significantly in roots in response to NH4+and glutamine directly. Our results provide new insights about GOGATs in poplar and their expression patterns under exogenous substances, to lay molecular basis for studying gene function and provide a reference for exploring putative roles of GOGATs in carbon-nitrogen balance. | |
| 650 | 1 | 4 | _aCARBON AND NITROGEN METABOLISM |
| 650 | 1 | 4 | _aEXPRESSION PATTERNS |
| 650 | 1 | 4 | _aGENE IDENTIFICATION |
| 650 | 1 | 4 | _aGLUTAMATE SYNTHASE |
| 650 | 1 | 4 | _aPOPLAR |
| 700 | 1 | 2 | _aCao, L. |
| 700 | 1 | 2 | _aXu, C. |
| 700 | 1 | 2 | _aSun, Y. |
| 700 | 1 | 2 | _aNiu, C. |
| 700 | 1 | 2 | _aLeng, X. |
| 700 | 1 | 2 | _aHao, B. |
| 700 | 1 | 2 | _aLiu, G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1bVp_8D1vsu4C1NYo5kgnaTLyJWodmnev/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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