| 000 | 02443nam a2200301Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625162441.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20006 | ||
| 245 | 1 | 0 | _aPIN5 is involved in regulating NH4 + efflux and primary root growth under high.ammonium stress via mediating intracellular auxin transport |
| 490 | 0 | _vPlant and Soil, 10.1007/s11104-023-05869-z, 2023 | |
| 520 | 3 | _aBackground and Aims Ammonium (NH4+)is an important nitrogen (N)source in many ecosystems and agricultural systems but excessive NH4 + is toxic to root growth and development, especially when NH4 + is the sole N source. Previous studies have shown that polar auxin (indole-3-acetic acid, IAA)transport mediated by PIN2 and AUX1 is critical for maintaining lateral root development under high- NH4 + stress. However, the regulation of subcellular IAA homeostasis under high-NH4 + stress has remained unclear. Methods Knockout mutants for the intracelular IAA transporter PIN5 and the plasma-membrane H+- ATPases AHA1 and AHA2 were used, and primary root length, transmembrane H+ fluxes, and NH4 + fluxes in the roots were determined. Results We show that high NH4 + disturbs the subcellular IAA homeostasis by upregulating the expression of PIN5. Knockout of PIN5 resulted in elevated cytoplastic IAA accumulation and reduced NH4 + efflux under high-NH4 + stress. Furthermore, we show that NH4 + treatment promotes H+ efflux at the root elongation zone, in turn promoting NH4 + efflux, with the involvement of PIN5-mediated intracelular IAA transport. Moreover, stabilization of rhizosphere pH reduced NH4 + efflux and promoted primary root growth in the pin5 mutant under high NH4 +. Conclusion Our findings provide a mechanistic explanation for the role of subcellular IAA homeostasis in response to high-NH4 + stress through the coordinated regulation of NH4 + efflux and H+ efflux. | |
| 650 | 1 | 4 | _aPIN5 |
| 650 | 1 | 4 | _aAMMONIUM |
| 650 | 1 | 4 | _aINTRACELLULAR AUXIN HOMEOSTASIS |
| 650 | 1 | 4 | _aH+ FLUXES |
| 650 | 1 | 4 | _aNH4+FLUXES |
| 700 | 1 | 2 | _aDi, D. W. |
| 700 | 1 | 2 | _aWu, J. |
| 700 | 1 | 2 | _aMa, M. |
| 700 | 1 | 2 | _aLi, G. |
| 700 | 1 | 2 | _aWang, M. |
| 700 | 1 | 2 | _aKronzucker, H. J. |
| 700 | 1 | 2 | _aShi, W. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1aaL3YfnydFZ3Jr3hJWpRzUor9pbh6cfF/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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