| 000 | 02278nam a2200229Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162441.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20010 | ||
| 245 | 1 | 0 | _aThe ability of Pichia kudriavzevii to tolerate multiple stresses makes it promising for developing improved bioethanol production processes |
| 490 | 0 | _vLetters in Applied MicroBiology, 75(1), p.36-44, 2022 | |
| 520 | 3 | _aThermotolerant ethanol fermenting yeasts have been extensively used in industrial bioethanol production. However, little is known about yeast physiology under stress during bioethanol processing. This study investigated the physiological characteristics of the thermotolerant yeast Pichia kudriavzevii, strains NUNS-4, NUNS-5 and NUNS-6, under the multiple stresses of heat, ethanol and sodium chloride. Results showed that NUNS-4, NUNS-5 and NUNS-6 displayed higher growth rates under each stress condition than the reference strain, Saccharomyces cerevisiae TISTR5606. Maximum specific growth rates under stresses of heat (45°C), 15 percent v/v ethanol and 1·0 M sodium chloride were 0·23 ± 0·04 (NUNS-4), 0·11 ± 0·01 (NUNS-5)and 0·15 ± 0·01 h-1 (NUNS-5), respectively. Morphological features of all yeast studied changed distinctly with the production of granules and vacuoles when exposed to ethanol, and cells were elongated under increased sodium chloride concentration. This study suggests that the three P. kudriavzevii strains are potential candidates to use in industrial-scale fermentation due to a high specific growth rate under multiple stress conditions. Multiple stress-tolerant P. kudriavzevii NUNS strains have received much attention not only for improving large-scale fuel ethanol production, but also for utilizing these strains in other biotechnological industries. | |
| 650 | 1 | 4 | _aMORPHOMETRIC CHARACTERIZATION |
| 650 | 1 | 4 | _aMULTI-STRESS TOLERANCE |
| 650 | 1 | 4 | _aSPECIFIC GROWTH RATE |
| 650 | 1 | 4 | _aSPOT DILUTION GROWTH ASSAY |
| 650 | 1 | 4 | _aYEAST VACUOLE DEVELOPMENT |
| 700 | 1 | 2 | _aPongcharoen, P. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1Id53vDot_ePw5i1iteb96b1AIq-3khPA/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c54115 _d54115 |
||