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090			_aB-20014
245	1	0	_aAntibacterial activity of chitosan grafting nisin: Preparation and characterization
490	0		_vReactive & Functional Polymers, 91, p.71-76, 2015
520	3		_aNisin grafted chitosan was prepared by using microbial transglutaminase as biocatalyst. The transglutaminase-catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The results revealed that the degree of substitution (DS)of nisin-chitosan could be controlled by adjusting the reaction time, the reaction temperature and the molar ratio of nisin to chitosan. And nisin-chitosan in different pH showed excellent solubility. In addition, in vitro antibacterial activity assessment, nisin-chitosan with the concentration of 0.008 mg/mL showed pronounced inhibitory effect against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis)and Gram-negative bacteria (Escherichia coli). Furthermore, L929 mouse fibroblasts were cultured with nisin-chitosan, and the methylthiazol tetrazolium (MTT)assay showed that nisin-chitosan with the concentration from 0.005 to 0.01 mg/mL displayed low toxicity. The results may contribute to finding the application of nisin-chitosan in pharmaceutical and food industry fields..
650	1	4	_aCHITOSAN
650	1	4	_aNISIN
650	1	4	_aMICROBIAL TRANSGLUTAMINASE
650	1	4	_aANTIBACTERIAL ACTIVITY
650	1	4	_aLOW TOXICITY
700	1	2	_aZhu, X.
700	1	2	_aWu, H.
700	1	2	_aYang, J.
700	1	2	_aTong, J.
700	1	2	_aYi, J.
700	1	2	_aHu, Z.
700	1	2	_aFan, L.
856	4	0	_uhttps://drive.google.com/file/d/1YBT0yfFh6hwCevPqjZRhHECEmnlfbgHY/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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