| 000 | 02416nam a2200289Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162445.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20258 | ||
| 245 | 1 | 0 | _aPlasmodesmal endoplasmic reticulum proteins regulate intercellular trafficking of cucumber mosaic virus in Arabidopsis |
| 490 | 0 | _vJournal of Experimental Botany, 74(15), p.4401-4414, 2023 | |
| 520 | 3 | _aPlasmodesmata (PD)are plasma membrane-lined cytoplasmic nanochannels that mediate cell-to-cell communication across the cell wall. A range of proteins are embedded in the PD plasma membrane and endoplasmic reticulum (ER), and function in regulating PD-mediated symplasmic trafficking. However, knowledge of the nature and function of the ER-embedded proteins in the intercellular movement of non-cell-autonomous proteins is limited. Here, we report the functional characterization of two ER luminal proteins, AtBiP1/2, and two ER integral membrane proteins, AtERdj2A/B, which are located within the PD. These PD proteins were identified as interacting proteins with cucumber mosaic virus (CMV)movement protein (MP)in co-immunoprecipitation studies using an Arabidopsis-derived plasmodesmal-enriched cell wall protein preparation (PECP). The AtBiP1/2 PD location was confirmed by TEM-based immunolocalization, and their AtBiP1/2 signal peptides (SPs)function in PD targeting. In vitro/in vivo pull-down assays revealed the association between AtBiP1/2 and CMV MP, mediated by AtERdj2A, through the formation of an AtBiP1/2-AtERdj2-CMV MP complex within PD. The role of this complex in CMV infection was established, as systemic infection was retarded in bip1/bip2w and erdj2b mutants. Our findings provide a model for a mechanism by which the CMV MP mediates cell-to-cell trafficking of its viral ribonucleoprotein complex. | |
| 650 | 1 | 4 | _aPLASMODESMATA (PD) |
| 650 | 1 | 4 | _aNON-CELL AUTONOMOUS PROTEIN (NCAP) |
| 650 | 1 | 4 | _aER-PROTEINS |
| 650 | 1 | 4 | _aVIRAL MOVEMENT PROTEIN |
| 650 | 1 | 4 | _aVIRUS INFECTION |
| 650 | 1 | 4 | _aCUCUMBER MOSAIC VIRUS (CMV) |
| 700 | 1 | 2 | _aHam, B. K. |
| 700 | 1 | 2 | _aWang, X. |
| 700 | 1 | 2 | _aToscano-Morales, R. |
| 700 | 1 | 2 | _aLin, J. |
| 700 | 1 | 2 | _aLucas, W. J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1jvDBJqREqVbd4xowcIzvgtahdMbwNAia/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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