| 000 | 02475nam a2200313Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162446.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20290 | ||
| 245 | 1 | 0 | _aThe transcription factor BnaWRKY10 regulates cytokinin dehydrogenase BnaCKX2 to control cytokinin distribution and seed size in Brassica napus |
| 490 | 0 | _vJournal of Experimental Botany, 10.1093/jxb/erad201, 2023 | |
| 520 | 3 | _aCytokinins (CKs)are the phytohormones that promote cell division and differentiation. However, the regulation of CKs distribution and homeostasis in Brassica napus is poorly understood. Here, the endogenous CKs were first quantified by LC-ESI-MS/MS in rapeseed tissues and visualized by TCSn::GUS reporter lines. Interestingly, the cytokinin oxidase/dehydrogenase BnaCKX2 homologs were mainly expressed in reproductive organs. Later, the quadruple mutants of the four BnaCKX2 homologs were generated. Endogenous CKs were increased in the seeds of the BnaCKX2 quadruple mutants, resulting in a significantly reduced seed size. In contrast, overexpression of BnaA9.CKX2 resulted in larger seeds, probably by delaying endosperm cellularization. Furthermore, BnaC6.WRKY10b, but not BnaC6.WRKY10a, positively regulated BnaA9.CKX2 expression by binding directly to its promoter region. Overexpression of BnaC6.WRKY10b rather than BnaC6.WRKY10a resulted in lower CKs levels and larger seeds by activating BnaA9.CKX2 expression, indicating that the functional differentiation of BnaWRKY10 homologs might have occurred during the B. napus evolution or domestication. Notably, the haploid types of BnaA9.CKX2 were associated with 1000-seed weight in the natural B. napus population. Overall, the study reveals the distribution of CKs in B. napus tissues, and highlights that the BnaWRKY10-mediated BnaCKX2 expression is essential for seed size regulation, providing promising targets for oil crop improvement. | |
| 650 | 1 | 4 | _aBNACKX2 |
| 650 | 1 | 4 | _aBNAWRKY10 |
| 650 | 1 | 4 | _aBRASSICA NAPUS |
| 650 | 1 | 4 | _aCYTOKININ |
| 650 | 1 | 4 | _aSEED SIZE |
| 650 | 1 | 4 | _aSEED WEIGHT |
| 700 | 1 | 2 | _aYan, G. |
| 700 | 1 | 2 | _aLi, S. |
| 700 | 1 | 2 | _aMa, M. |
| 700 | 1 | 2 | _aQuan, C. |
| 700 | 1 | 2 | _aTian, X. |
| 700 | 1 | 2 | _aTu, J. |
| 700 | 1 | 2 | _aDai, C. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1nxbLNcU2BSXzpz0TnzRcux6RN1LPSmSA/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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