000			02019nam a2200253Ia 4500
003			MX-MdCICY
005			20250625162446.0
040			_cCICY
090			_aB-20322
245	1	0	_aPlastid engineering using episomal DNA
490	0		_vPlant Cell Reports, 42, p.1125-1132, 2023
520	3		_aPlastids represent valuable subcellular compartments for genetic engineering of plants with intrinsic advantages to engineering the nucleus. The ability to perform site-specific transgene integration by homologous recombination (HR), coordination of transgene expression in operons, and high production of heterologous proteins, all make plastids an attractive target for synthetic biology. Typically, plastid engineering is performed by homologous recombination; however, episomal-replicating vectors have the potential to accelerate the design/build/test cycles for plastid engineering. By accelerating the timeline from design to validation, it will be possible to generate translational breakthroughs in fields ranging from agriculture to biopharmaceuticals. Episomal-based plastid engineering will allow precise single step metabolic engineering in plants enabling the installation of complex synthetic circuits with the ambitious goal of reaching similar efficiency and flexibility of to the state-of-the-art genetic engineering of prokaryotic systems. The prospect to design novel episomal systems for production of transplastomic marker-free plants will also improve biosafety for eventual release in agriculture
650	1	4	_aMINI-SYNPLASTOME
650	1	4	_aMINICHROMOSOME
650	1	4	_aEPISOMAL REPLICATION
650	1	4	_aCHLOROPLAST ORI
650	1	4	_aGEMINIVIRUS REP SYSTEM
650	1	4	_aPLASTID ENGINEERING
700	1	2	_aOcchialini, A.
700	1	2	_aLenaghan, S. C.
856	4	0	_uhttps://drive.google.com/file/d/1CxV7wnl1VHF03Pu6N1TCtgJB7f13Ick3/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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