| 000 | 02564nam a2200337Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625162448.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20397 | ||
| 245 | 1 | 0 | _aStrigolactones promote the localization of the ABA exporter ABCG25 at the plasma membrane in root epidermal cells of Arabidopsis thaliana |
| 490 | 0 | _vJournal of Experimental Botany, 74(18), p.5881-5895, 2023 | |
| 520 | 3 | _aThe phytohormones strigolactones crosstalk with abscisic acid (ABA)in acclimation to osmotic stress, as ascertained in leaves. However, our knowledge about underground tissues is limited, and lacking in Arabidopsis: whether strigolactones affect ABA transport across plasma membranes has never been addressed. We evaluated the effect of strigolactones on the localization of ATP BINDING CASSETTE G25 (ABCG25), an ABA exporter in Arabidopsis thaliana. Wild-type, strigolactone-insensitive, and strigolactone-depleted seedlings expressing a green fluorescent protein:ABCG25 construct were treated with ABA or strigolactones, and green fluorescent protein was quantified by confocal microscopy in different subcellular compartments of epidermal root cells. We show that strigolactones promote the localization of an ABA transporter at the plasma membrane by enhancing its endosomal recycling. Genotypes altered in strigolactone synthesis or perception are not impaired in ABCG25 recycling promotion by ABA, which acts downstream or independent of strigolactones in this respect. Additionally, we confirm that osmotic stress decreases strigolactone synthesis in A. thaliana root cells, and that this decrease may support local ABA retention under low water availability by allowing ABCG25 internalization. Thus, we propose a new mechanism for ABA homeostasis regulation in the context of osmotic stress acclimation: the fine-tuning by strigolactones of ABCG25 localization in root cells. | |
| 650 | 1 | 4 | _aARABIDOPSIS THALIANA |
| 650 | 1 | 4 | _aABCG25 |
| 650 | 1 | 4 | _aABSCISIC ACID |
| 650 | 1 | 4 | _aGR245DS |
| 650 | 1 | 4 | _aENDOCYTOSIS |
| 650 | 1 | 4 | _aOSMOTIC STRESS |
| 650 | 1 | 4 | _aROOTS |
| 650 | 1 | 4 | _aSTRIGOLACTONES |
| 700 | 1 | 2 | _aRusso, G. |
| 700 | 1 | 2 | _aCapitanio, S. |
| 700 | 1 | 2 | _aTrasoletti, M. |
| 700 | 1 | 2 | _aMorabito, C. |
| 700 | 1 | 2 | _aKorwin Krukowski, P. |
| 700 | 1 | 2 | _aVisentin, I. |
| 700 | 1 | 2 | _aCardinale, F. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1ozOoGXIbSkzlN2c2BGz5JcfXXG45cvtT/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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