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245	1	0	_aA wound inducible chalcone synthase gene from Dysoxylum gotadhora (DbCHS)regulates flavonoid biosynthesis
490	0		_vPhysiology and Molecular Biology of Plants, 29(7), p.959-969, 2023
520	3		_aChalcone synthase (CHS)is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects. In this study, we used the leaves of D. gotadhora to clone CHS gene (DbCHS). The 1188-bp open reading frame (ORF)was part of the 1373-bp full-length DbCHS clone. Compared to other parts of the plant, DbCHS is expressed more in the leaves and fruits. This is linked to anti-microbial action against a panel of microbes in these tissues. The leaves and seeds extracts inhibit Bacillus subtilis, Streptococcus pyogenes, Bacillus cereus, and Candida albicans. When a plant is hurt, it leaves its tissues open to attack by microbes. To protect themselves, plants often make chemicals that kill microbes. We found that wounding had a big effect on the production of DbCHS. Based on these tests and the results of phylogenetic analysis and molecular docking, we believe that DbCHS is a wound-inducible enzyme that is needed to make flavonoids, which may give the plant antimicrobial properties.
650	1	4	_aANTIMICROBIAL ACTIVITY
650	1	4	_aCHALCONE SYNTHASE
650	1	4	_aDYSOXYLUM BINECTARIFERUM
650	1	4	_aDYSOXYLUM GOTADHORA
650	1	4	_aFLAVONOIDS
650	1	4	_aSECONDARY METABOLITES
700	1	2	_aMahajan, V.
700	1	2	_aChouhan, R.
700	1	2	_aJamwal, V. L.
700	1	2	_aKapoor, N.
700	1	2	_aGandhi, S. G.
856	4	0	_uhttps://drive.google.com/file/d/1hhdkR0YnUN3Ai8SSq6zAKHM29BTAPkFU/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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