| 000 | 02361nam a2200241Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625162450.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20543 | ||
| 245 | 1 | 0 | _amiR171-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 regulate somatic embryogenesis in citrus |
| 490 | 0 | _vPlant Physiology, 192, p.2838-2854, 2023 | |
| 520 | 3 | _aSomatic embryogenesis (SE)is a key regeneration pathway in various biotechnology approaches to crop improvement, especially for economically important perennial woody crops like citrus. However, maintenance of SE capability has long been a challenge and becomes a bottleneck in biotechnology-facilitated plant improvement. In the embryogenic callus (EC)of citrus, we identified 2 csi-miR171c-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 (CsSCL2/3), which exert positive feedback regulation on csi-miR171c expression. Suppression of CsSCL2 expression by RNA interference (RNAi)enhanced SE in citrus callus. A thioredoxin superfamily protein CsClot was identified as an interactive protein of CsSCL2/3. Overexpression of CsClot disturbed reactive oxygen species (ROS)homeostasis in EC and enhanced SE. Chromatin immunoprecipitation sequencing (ChIP-Seq)and RNA-Seq identified 660 genes directly suppressed by CsSCL2 that were enriched in biological processes including development-related processes, auxin signaling pathway, and cell wall organization. CsSCL2/3 bound to the promoters of regeneration-related genes, such as WUSCHEL-RELATED HOMEOBOX 2 (CsWOX2), CsWOX13, and Lateral Organ Boundaries Domain 40 (LBD40), and repressed their expression. Overall, CsSCL2/3 modulate ROS homeostasis through the interactive protein CsClot and directly suppress the expression of regeneration-related genes, thus regulating SE in citrus. We uncovered a regulatory pathway of miR171c-targeted CsSCL2/3 in SE, which shed light on the mechanism of SE and regeneration capability maintenance in citrus. | |
| 700 | 1 | 2 | _aFeng, M. Q. |
| 700 | 1 | 2 | _aJiang, N. |
| 700 | 1 | 2 | _aWang, P. B. |
| 700 | 1 | 2 | _aLiu, Y. |
| 700 | 1 | 2 | _aXia, Q. M. |
| 700 | 1 | 2 | _aJia, H. H. |
| 700 | 1 | 2 | _aWu, X. M. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1xRUc9fyLO6SnYChNvGCHD1FkSRM6zA0N/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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