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245 1 0 _aPlant protoplasts in the age of synthetic biology
490 0 _vJournal of Experimental botany, 74(13), p.3821-3832, 2023
520 3 _aProtoplasts, which are plant cells with their cell walls removed, have been used for decades in plant research and have been instrumental in genetic transformation and the study of various aspects of plant physiology and genetics. With the advent of synthetic biology, these individualized plant cells are fundamental to accelerate the 'design-build-test-learn' cycle, which is relatively slow in plant research. Despite their potential, challenges remain in expanding the use of protoplasts in synthetic biology. The capacity of individual protoplasts to hybridize to form new varieties, and to regenerate from single cells, creating individuals with new features is underexplored. The main objective of this review is to discuss the use of protoplasts in plant synthetic biology and to highlight the challenges to exploiting protoplast technologies in this new 'age of synthetic biology'.
650 1 4 _aCELL WALL DIGESTION
650 1 4 _aGENETIC CIRCUITS
650 1 4 _aGENETIC TRANSFORMATION
650 1 4 _aMINIATURIZATION
650 1 4 _aPROTOPLASTS
650 1 4 _aREGENERATION
650 1 4 _aSYNTHETIC BIOLOGY
700 1 2 _aReyna-Llorens, I.
700 1 2 _aFerro-Costa, M.
700 1 2 _aBurgess, S. J.
856 4 0 _uhttps://drive.google.com/file/d/1IVPw__BrsKN7BucMiWhud_kBe0j_2ThZ/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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