000			02620nam a2200349Ia 4500
003			MX-MdCICY
005			20250625162451.0
040			_cCICY
090			_aB-20563
245	1	0	_aIsolation and characterization of barley (Hordeum vulgare)extracellular vesicles to assess their role in RNA spray-based crop protection
490	0		_vInternational Journal of Molecular Sciences, 22(13), p.7212, 2021
520	3		_aThe demonstration that spray-induced gene silencing (SIGS)can confer strong disease resistance, bypassing the laborious and time-consuming transgenic expression of double-stranded (ds)RNA to induce the gene silencing of pathogenic targets, was ground-breaking. However, future field applications will require fundamental mechanistic knowledge of dsRNA uptake, processing, and transfer. There is increasing evidence that extracellular vesicles (EVs)mediate the transfer of transgene-derived small interfering (si)RNAs in host-induced gene silencing (HIGS)applications. In this study, we establish a protocol for barley EV isolation and assess the possibilities for EVs regarding the translocation of sprayed dsRNA from barley (Hordeum vulgare)to its interacting fungal pathogens. We found barley EVs that were 156 nm in size, containing predominantly 21 and 19 nucleotide (nts)siRNAs, starting with a 5'-terminal Adenine. Although a direct comparison of the RNA cargo between HIGS and SIGS EV isolates is improper given their underlying mechanistic differences, we identified sequence-identical siRNAs in both systems. Overall, the number of siRNAs isolated from the EVs of dsRNA-sprayed barley plants with sequence complementarity to the sprayed dsRNA precursor was low. However, whether these few siRNAs are sufficient to induce the SIGS of pathogenic target genes requires further research. Taken together, our results raise the possibility that EVs may not be mandatory for the spray-delivered siRNA uptake and induction of SIGS
650	1	4	_aFUSARIUM GRAMINEARUM
650	1	4	_aRNA INTERFERENCE
650	1	4	_aRNA SPRAY
650	1	4	_aRNAI
650	1	4	_aBARLEY
650	1	4	_aDSRNA
650	1	4	_aEXTRACELLULAR VESICLES
650	1	4	_aPLANT EV
650	1	4	_aSIRNA
700	1	2	_aSchlemmer, T.
700	1	2	_aBarth, P.
700	1	2	_aWeipert, L.
700	1	2	_aPreußer, C.
700	1	2	_aHardt, M.
700	1	2	_aMöbus, A.
700	1	2	_aKoch, A.
856	4	0	_uhttps://drive.google.com/file/d/1uQgA3jBJXR_0i4tOLdhZ54ZTqJpUVLGx/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c54650 _d54650