| 000 | 02407nam a2200301Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625162452.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-20626 | ||
| 245 | 1 | 0 | _aGenome-wide characterization of NBS-LRR family genes and expression analysis under powdery mildew stress in Lagenaria siceraria |
| 490 | 0 | _vPhysiological and Molecular Plant Pathology, 118, p.101798, 2022 | |
| 520 | 3 | _aAs the largest group of plant resistance genes, the nucleotide-binding site-leucine-rich repeat (NBS-LRR)genes play a critical role in plant disease defense system. The bottle gourd is a distinctive horticultural crop of the Cucurbitaceae family and powdery mildew is one of the major diseases jeopardizing crop production and quality. In this study, we have identified 84 NBS-LRR genes and divided into seven subfamilies. Phylogenetic tree and motif analysis grouped these genes into two major clusters with toll/interleukin-1 like receptor (TIR)domain or a coiled-coil domain. Subsequently, duplication events, motif sequence, orthologous gene cluster, protein-protein interaction, cis-elements were analyzed. 12 pairs of tandem duplicated genes and two pairs of segmental duplicated genes were detected in NBS-LRR family. 14 singleton genes and 62 functional cis-elements were found. The structure of 27 proteins were constructed by homology modeling and divided into five different types. 34 differentially expressed genes of NBS-LRR genes were found in PM-resistant and PM-susceptible line. Expression of NBS-LRR genes were validated by qRT-PCR under PM stress and gene Lsi04g015960 with powdery mildew8 (RPW8)domain was significantly up-regulated in PM-resistant variety. Our study provides fundamental insights of NBS-LRR family genes in bottle gourd and find a potential candidate gene Lsi04g015960 for PM tolerance breeding. | |
| 650 | 1 | 4 | _aBOTTLE GOURD |
| 650 | 1 | 4 | _aNBS-LRR FAMILY |
| 650 | 1 | 4 | _aPHYLOGENETIC ANALYSIS |
| 650 | 1 | 4 | _aPOWDERY MILDEW |
| 650 | 1 | 4 | _aEXPRESSION ANALYSIS |
| 700 | 1 | 2 | _aWang, J. |
| 700 | 1 | 2 | _aYang, C. |
| 700 | 1 | 2 | _aWu, X. |
| 700 | 1 | 2 | _aWang, Y. |
| 700 | 1 | 2 | _aWang, B. |
| 700 | 1 | 2 | _aWu, X. |
| 700 | 1 | 2 | _aLi, G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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