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040			_cCICY
090			_aB-20630
245	1	0	_aCreation of cardiac tissue exhibiting mechanical integration of spheroids using 3D bioprinting
490	0		_vJoVE (Journal of Visualized Experiments), 125, p.e55438, 2017
520	3		_aThis protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array. Three days after 3D bioprinting, the spheroids are removed as an intact patch, which is already spontaneously beating. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
650	1	4	_aBIOENGINEERING
650	1	4	_aISSUE 125
650	1	4	_aCARDIAC TISSUE ENGINEERING
650	1	4	_a3D PRINTING
650	1	4	_aBIOPRINTING
650	1	4	_aBIOFABRICATION
650	1	4	_aADDITIVE MANUFACTURING
650	1	4	_aHEART FAILURE
650	1	4	_aHUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES
700	1	2	_aOng, C. S.
700	1	2	_aFukunishi, T.
700	1	2	_aNashed, A.
700	1	2	_aBlazeski, A.
700	1	2	_aZhang, H.
700	1	2	_aHardy, S.
700	1	2	_aHibino, N.
856	4	0	_uhttps://drive.google.com/file/d/1bwsVTrrtRzwL0f0wCrqbz8gdS-q4fj3s/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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