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245 1 0 _aThymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms
490 0 _vLetters in Applied MicroBiology, 74(5), p.787-795, 2022
520 3 _aMethicillin-resistant Staphylococcus aureus (MRSA)is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO)against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC)and the minimum lethal concentration (MLC)of the T. capitata EO was first investigated on a group of clinical isolates from septicaemias, diabetic foot ulcers and osteomyelitis. Biofilms were incubated with the EO at the MLC and its anti-biofilm potential was investigated. A strong antimicrobial activity was observed, with MIC and MLC values between 0·32 and 0·64 mg l-1 . However, the concentration of EO necessary for the eradication of planktonic cells was insufficient to significantly reduce the biofilm biomass of some isolates. Nevertheless, cell culturability and overall cellular metabolism was strongly reduced in all biofilms tested, only when the EO was tested. Contrary to the tested antibiotics, T. capitata EO showed a significant antimicrobial activity against MRSA biofilms, by reducing cellular metabolism and cellular culturability.
650 1 4 _aEO
650 1 4 _aMRSA
650 1 4 _aXTT
650 1 4 _aBIOFILM BIOMASS
650 1 4 _aCELL CULTURABILITY
650 1 4 _aCELLULAR ACTIVITY
650 1 4 _aMINIMUM INHIBITORY CONCENTRATION
650 1 4 _aMINIMUM LETHAL CONCENTRATION
700 1 2 _aAlmeida, L.
700 1 2 _aLopes, N.
700 1 2 _aGaio, V.
700 1 2 _aCavaleiro, C.
700 1 2 _aSalgueiro, L.
700 1 2 _aSilva, V.
700 1 2 _aCerca, N.
856 4 0 _uhttps://drive.google.com/file/d/18OnYT1IcP5GjENV8oQhxSENt214JkyeZ/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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