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245	1	0	_aScopolamine production by root cultures of Duboisia myoporoides: II. Establishment of a hairy root culture by infection with Agrobacterium rhizogenes
490	0		_vJournal of Plant physiology, 131(3-4), p.315-323, 1987
520	3		_aA hairy root culture of Duboisia myoporoides was established by transforming cultured shoots with Agrobacterium rhizogenes strain HRI. Approximately one month after inoculation with HRI, a shoot produced many roots at the inoculation point. The roots, designated AR-4, were transferred to Linsmaier-Skoog liquid medium without growth regulators and were sub-cultured every three weeks. Opines, agropine and mannopine were detected in the extract of AR-4 cells. The scopolamine content of AR-4 was lower than that of untransformed roots (RB-1), whereas the hyoscyamine content was higher. Cell division took place during the culture of AR-4; the cell yield of AR-4 was 2-fold that of RB-1 after three weeks of culture. The concentration of endogenous indoleacetic acid in the AR-4 cells was not very high as compared with that of the RB-1 cells. The addition of indoleacetic acid or indolebutyric acid to the medium had little effect on the production of tropane alkaloids by AR-4, whereas 2,4-dichlorophenoxyacetic acid and benzyladenine decreased alkaloid production.
650	1	4	_aDUBOISIA MYOPOROIDES
650	1	4	_aAGROBACTERIUM RHIZOGENES
650	1	4	_aHAIRY ROOT
650	1	4	_aAGROPIR:E
650	1	4	_aMANNOPINE
650	1	4	_aSCOPOLAMINE
650	1	4	_aBYOSCYAMINE
650	1	4	_aINDOLEACETIC ACID
700	1	2	_aDeno, H.
700	1	2	_aYamagata, H.
700	1	2	_aEmoto, T.
700	1	2	_aYoshioka, T.
700	1	2	_aYamada, Y.
700	1	2	_aFujita, Y.
856	4	0	_uhttps://drive.google.com/file/d/11dQrs9OUdXemTL-KY9ShGN28bprBCVvq/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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