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245	1	0	_aGlucose oxidase as an important yet overlooked factor determining the antibacterial activity of bee pollen and bee bread
490	0		_vEuropean Food Research and Technology, 248(12), p.2929-2939, 2022
520	3		_aBee pollen (BP)and bee bread (BB)have attracted great attention due to their biological activities including antibacterial activity. However, the mechanism of antibacterial activity is largely unknown. Therefore, we aimed to characterise the antibacterial effect of BP and BB aqueous extracts against bacterial pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and Enterococcus faecalis)and identify the key compound(s)responsible for this effect. Here, we demonstrate that BP and particularly BB extracts display antibacterial activity which is significantly increased in the presence of glucose. Immunoblot analysis of extracts revealed the presence of MRJP1 in all analysed BP and BB samples and the enzyme glucose oxidase (GOX)in the majority of BB samples. Treatment of extracts with catalase resulted in the restoration of bacterial growth but only in those samples where glucose supplementation caused the enhancement of antibacterial activity. Our findings provide a deeper understanding of antibacterial activity of BP/BB which is mediated by the enzymatic activity of bee-derived GOX.
650	1	4	_aBEE PROTEINS
650	1	4	_aMRJP
650	1	4	_aANTIBACTERIAL ACTIVITY
650	1	4	_aENZYMATIC ACTIVITY
650	1	4	_aBACTERIAL PATHOGEN
700	1	2	_aPelka, K.
700	1	2	_aBucekova, M.
700	1	2	_aGodocikova, J.
700	1	2	_aSzweda, P.
700	1	2	_aMajtan, J.
856	4	0	_uhttps://drive.google.com/file/d/1b7thmF7fzuhi7MYM0b9lvhbKEcQyIeOD/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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