000			01818nam a2200241Ia 4500
003			MX-MdCICY
005			20250625162454.0
040			_cCICY
090			_aB-20712
245	1	0	_aHeterologous expression and characterization of Penicillium citrinum nuclease P1 in Aspergillus niger and its application in the production of nucleotides
490	0		_vProtein Expression and Purification, 156, p.36-43, 2019
520	3		_aNuclease P1 gene (nuc P1)which was cloned from Penicillium citrinum and expressed in A percent niger Bdel4 with the low-background extracellular protein percent The expression strategy of multi-copy nuc P1 in the A percent niger with the linker of 2A peptide was applied to improve the enzyme activity of nuclease P1, the highest activity up to 77 percent6 U/mL percent After Ni-chelate purification, the specific enzyme activity, the optimum temperature and pH were 32 percent4 U/mg, 65 °C and 5 percent3 respectively percent The recombination nuclease P1 was activated by addition of Mg2+, Zn2+ and Cu2+, and inhibited by addition of Ca2+, Fe2+, Mn2+, Ni2+, Co2+, Mg2+, K+ and EDTA percent Furthermore, the enzyme hydrolyses yeast RNA efficiently into 5'- nucleotides percent Through enzymolysis, the highest concentration of nucleotides achieved 15 percent12 mg/mL, and 75U nuclease P1 is suitable amount should be added to the enzymolysis system percent
650	1	4	_aNUCLEASE P1
650	1	4	_a2A PEPTIDE
650	1	4	_aASPERGILLUS NIGER
650	1	4	_aRECOMBINANT EXPRESSION
700	1	2	_aChen, X.
700	1	2	_aWang, B.
700	1	2	_aPan, L.
856	4	0	_uhttps://drive.google.com/file/d/1cdWXN6i5MW7SVTdg7BpPxfXHslcZx-zd/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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