000			01786nam a2200265Ia 4500
003			MX-MdCICY
005			20250625162454.0
040			_cCICY
090			_aB-20755
245	1	0	_aRegeneration of a transgenic woody legume (Robinia pseudoacacia L., black locust)and morphological alterations induced by Agrobacterium rhizogenes-mediated transformation
490	0		_vPlant Science, 88(2), p.149-157, 1993
520	3		_aA repeatable and fast transformation-regeneration system has been developed for a woody legume, black locust (Robinia pseudoacacia L.). Hairy roots were produced from hypocotyl segments inoculated with Agrobacterium rhizogenes R1601, a super-virulent strain with eukaryotic kanamycin resistance gene, in less than 1 week and resulted in subsequent shoot regeneration within 4 weeks. Transgenic black locust trees were established in soil 12 weeks after inoculation with the A. rhizogenes strain. The hairy root phenotype found in regenerated plants, which has been characterized as having wrinkled leaves and abundant root development, became evident after 3 months of growth. Southern blot analysis clearly showed multiple integration of T-DNA (transferred DNA)and the presence of neomycin phosphotransferase (NPTII)gene sequences in the transformed black locust genome.
650	1	4	_aAGROBACTERIUM
650	1	4	_aBLACK LOCUST
650	1	4	_aHAIRY ROOT
650	1	4	_aLEGUME
650	1	4	_aTRANSFORMATION
700	1	2	_aHan, K. H.,
700	1	2	_aKeathley, D. E.
700	1	2	_aDavis, J. M.
700	1	2	_aGordon, M. P.
856	4	0	_uhttps://drive.google.com/file/d/1fPVJVT2XjnoGuZGjzWS-HuEsvoTXk7vU/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c54836 _d54836