000			01662nam a2200205Ia 4500
003			MX-MdCICY
005			20250625162456.0
040			_cCICY
090			_aB-20822
245	1	0	_aGeranylgeraniol potently induces caspase-3-like activity during apoptosis in human leukemia U937 cells
490	0		_vBioChemical and biophysical Research Communications, 234(3), p.641-645, 1997
520	3		_aIn a previous study, we showed that geranylgeraniol (GGO)is a potent inducer of apoptosis in human leukemia cells. The present study describes the effects of GGO on the activity of cysteine-dependent aspartate-directed proteases (caspases)in human leukemia U937 cells. The caspase-3 (CPP32)activity was increased in a time-dependent manner by treatment with 50 microM GGO, whereas no activation of caspase-1 (interleukin-1beta converting enzyme (ICE)) was observed in any time period under the same experimental conditions. Other isoprenyl compounds such as geraniol, geranylgerany-lacetone, and vitamin K2 had no measurable effects on the activities of either caspase-3 or caspase-1. A inhibitor that preferentially inhibits the caspase-3 related caspases, Z-DEVD-FMK, strongly blocked the GGO-induced DNA fragmentation. These results suggest the involvement of caspase-3 in GGO-induced apoptosis in U937 human leukemia cells.
700	1	2	_aMasuda, Y.
700	1	2	_aNakaya, M.
700	1	2	_aNakajo, S.
700	1	2	_aNakaya, K.
856	4	0	_uhttps://drive.google.com/file/d/1NSpOtpMT9qe_mtz-4Dd7frPDzz0n5Dx_/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c54902 _d54902