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090			_aB-20872
245	1	0	_aDirect somatic embryogenesis and plant regeneration from immature embryos of chilli (Capsicum annuum L.).
490	0		_vPlant Science, 89(1), p.107-112, 1993
520	3		_aPepper (cv. New Mexico - 6 and Rajur Hirapur)plants were regenerated from immature zygotic embryos via direct somatic embryogenesis. Somatic embryos were formed directly, without any intervening callus, on the zygotic embryo apex, embryo axis and cotyledons on Murashige and Skoog's (MS)medium containing 2,4-D (418 ?M), thidiazuron (10 ?M)and a high concentration of sucrose (6-10 percent). The best response was observed on MS medium containing 2,4-D (9 ?M), coconut water (10 percent)and high sucrose (8 percent). The entire process of induction and maturation of the embryos was completed on the same medium. Histological examination indicated that secondary embryogenesis also occurred directly from the primary somatic embryos. Differentiation of embryos was nonsynchronous, and some embryos were swollen and distorted with fasciation. More than 70 percent of the mature normal somatic embryos germinated readily on MS medium containing GA3 or TDZ, alone and in combination, and following transfer to pots developed into normal plants.
650	1	4	_aDIRECT SOMATIC EMBRYOGENESIS
650	1	4	_aCAPSICUM ANNUUM L.
650	1	4	_aIMMATURE EMBRYOS
650	1	4	_aPLANT REGENERATION
700	1	2	_aHarini, I.
700	1	2	_aSita, G. L.
856	4	0	_uhttps://drive.google.com/file/d/1eEs9HNhngclyDU9fd45UXKtDaSl0QMcs/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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