000			01992nam a2200217Ia 4500
003			MX-MdCICY
005			20250625162457.0
040			_cCICY
090			_aB-20875
245	1	0	_aProtein localization and interaction studies in plants: toward defining complete proteomes by visualization
490	0		_vAdvances in virus Research, 100, p.117-144, 2018
520	3		_aProtein interaction and localization studies in plants are a fundamental component of achieving mechanistic understanding of virus:plant interactions at the systems level. Many such studies are conducted using transient expression assays in leaves of Nicotiana benthamiana, the most widely used experimental plant host in virology, examined by laser-scanning confocal microscopy. This chapter provides a workflow for protein interaction and localization experiments, with particular attention to the many control and supporting assays that may also need to be performed. Basic principles of microscopy are introduced to aid researchers in the early stages of adding imaging techniques to their experimental repertoire. Three major types of imaging-based experiments are discussed in detail: (i)protein localization using autofluorescent proteins, (ii)colocalization studies, and (iii)bimolecular fluorescence complementation, with emphasis on judicious interpretation of the data obtained from these approaches. In addition to establishing a general framework for protein localization experiments in plants, the need for proteome-scale localization projects is discussed, with emphasis on nuclear-localized proteins.
650	1	4	_aCOLOCALIZATION
650	1	4	_aCONFOCAL MICROSCOPY
650	1	4	_aNICOTIANA BENTHAMIANA
650	1	4	_aPROTEIN INTERACTION
700	1	2	_aGoodin, M. M.
856	4	0	_uhttps://drive.google.com/file/d/1lg9k6s40C_BVpo7-4wxcRDY62gJM1NRD/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c54955 _d54955