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090			_aB-20910
245	1	0	_aProtocol for in vitro propagation of Excoecaria agallocha L., a medicinally important mangrove species
490	0		_vPlant Cell Reports, 17, p.861-865, 1998
520	3		_aAn in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node of binodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on a newly formulated medium containing BA, Zeatin and IBA in concentrations of 13.3 ?M, 4.65 ?M and 1.23 ?M, respectively. The new medium, first used in this study, has a specific composition of major nutrients, MS micronutrients and iron compounds. Nodal segments from rooted cuttings and seedlings responded better than those of mature tree explants. Multiple shoot induction was complemented with efficient shoot elongation, and repeated subculture of binodal segments from axillary shoots resulted in 10-12 shoots per explant in 3 months. Rooting was achieved by growing shoots in the new medium with 0.23 ?M IBA. Regenerated plants were successfully acclimatized to the natural environment, and about 85 percent of plantlets survived under ex vitro conditions. This is the first report of micropropagation in the genus Excoecaria and also in mangrove tree species.
650	1	4	_aEUPHORBIACEAE
650	1	4	_aEXCOECARIA AGALLOCHA
650	1	4	_aIN VITRO CULTURE
650	1	4	_aKEY WORDS CONSERVATION
650	1	4	_aMANGROVES
650	1	4	_aMEDICINAL PLANTS
650	1	4	_aMICROPROPAGATION
700	1	2	_aSrinivasa Rao, C.
700	1	2	_aEganathan, P.
700	1	2	_aAnand, A.
700	1	2	_aBalakrishna, P.
700	1	2	_aReddy, T. P.
856	4	0	_uhttps://drive.google.com/file/d/1Ck_mBGRMYaEYd79D_K4okgqY973MXUBH/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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