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| 090 | _aB-21012 | ||
| 245 | 1 | 0 | _aComparison of physical-chemical properties of type I collagen from different species |
| 490 | 0 | _vFood Chemistry, 99(2), p.244-251, 2006 | |
| 520 | 3 | _aType I collagen is an important biopolymer and has been widely used in biomaterials due to its excellent biocompatibility and biodegradable properties. However, only a few studies have been reported on its comparison in different species. The amino acid composition, SDS-PAGE, UV-Vis spectrum, thermal transition temperatures, extractable uronic acid/protein ratio and enzymatic sensitivity of type I collagen from bird feet (BF), bovine skin (BS), frog skin (FS), porcine skin (PS)and shark skin (SS)were evaluated. The amino acid composition of type I collagens were different from different species, BF collagen contained higher glutamic acid (Glu)and aspartic acid (Asp), SS collagen contained lower aspartic acid and hydroxyproline (Hyp). Similar SDS-PAGE profiles were found from different animal's collagen, all samples were composed of two a1-chain and one a2-chain. All UV-Vis spectrums exhibited a typical absorption peak at 218 nm. The UV absorption spectrum of BF collagen ranged from 190 to 340 nm, FS collagen ranged from 190 to 270 nm; the other species collagen ranged from 190 to 240 nm. Thermal transition temperatures of type I collagen from different animals decreased in the order of BF > BS > PS > FS > SS. PS collagen had higher extractable uronic acid/protein ratio and the lowest enzymatic sensitivity. Summarizing these results, the BF collagen had higher hyproxyproline (Hyp)+ proline (Pro)value and exhibited higher thermal stability; the PS collagen contained larger amount of glycosaminoglycan and resulted in a high enzymes resistance. However, the BF and PS collagen should be used as a suitable material in biomaterial utilitys because of its better biostability. | |
| 650 | 1 | 4 | _aPHYSICAL-CHEMICAL PROPERTIES |
| 650 | 1 | 4 | _aSPECIES |
| 650 | 1 | 4 | _aCOLLAGEN |
| 700 | 1 | 2 | _aLin, Y. K. |
| 700 | 1 | 2 | _aLiu, D. C. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/open?id=1umh4xVtGlDGlQo-5B2oeB4V09wQUx85-&usp=drive_copy _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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