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090			_aB-21055
245	1	0	_aIncreasing activity of the GS-GOGAT cycle highlights the compensation of N-assimilation in the absence of nitrogen and its metabolic effects in cyanobacteria
490	0		_vAlgal Research, 79, p.103490, 2024
520	3		_aComparative studies of proteome analysis, photosynthetic activity and fatty acid biosynthesis of the glnA- and glsF-overexpressing strains and the wild-type (WT)were carried out to elucidate the possible role(s)of the two enzymes, glutamine synthetase (GS)and glutamate synthase (GOGAT)in the GS-GOGAT cycle under nitrogen starvation. The evidence indicated the role of GS and GOGAT in the regulatory mechanism of carbon (C-)and nitrogen (N-)assimilation and the induction of photosynthesis and lipid/fatty acid biosynthesis pathway under N-stress. The fatty acid profile of the GS-overexpressing strain (WT + GlnA)under the stress showed a drastic increase in the levels of C18:1?9 and C18:1?11, whereas only C18:1?9 was significantly induced in the GOGAT-overexpressing strain (WT + GlsF). Moreover, the proteome analysis and oxygen evolution data showed that the extra-GS activity in the mutant (MT)cells led to the increasing of proteins involved in photosynthesis as well as the rate of oxygen evolution in the first 24 h after nitrogen depletion, suggesting its role in promoting conversion of light energy to chemical energy under the stress.
650	1	4	_aENZYMATIC ACTIVITY
650	1	4	_aARTHROSPIRA PLATENSIS
650	1	4	_aSYNECHOCOCCUS ELONGATUS PCC 7942
650	1	4	_aFATTY ACID BIOSYNTHESIS
650	1	4	_aPHOTOSYNTHESIS
650	1	4	_aNITROGEN DEPLETION
700	1	2	_aYutthanasirikul, R.
700	1	2	_aKurdrid, P.
700	1	2	_aSaree, S.
700	1	2	_aSenachak, J.
700	1	2	_aSaelee, M.
700	1	2	_aHongsthong, A.
856	4	0	_uhttps://drive.google.com/open?id=1hg7uN__msfbNtBdt9ZOvNUOcTd2LS2TD&usp=drive_copy _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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