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090			_aB-21112
245	1	0	_aCsRAXs negatively regulate leaf size and fruiting ability through auxin glycosylation in cucumber
490	0		_vJournal of Integrative Plant Biology, 10.1111/jipb.13655, 2024
520	3		_aLeaves are the main photosynthesis organ that directly determines crop yield and biomass. Dissecting the regulatory mechanism of leaf development is crucial for food security and ecosystem turn-over. Here, we identified the novel function of R2R3-MYB transcription factors CsRAXs in regulating cucumber leaf size and fruiting ability. Csrax5 single mutant exhibited enlarged leaf size and stem diameter, and Csrax1/2/5 triple mutant displayed further enlargement phenotype. Overexpression of CsRAX1 or CsRAX5 gave rise to smaller leaf and thinner stem. The fruiting ability of Csrax1/2/5 plants was significantly enhanced, while that of CsRAX5 overexpression lines was greatly weakened. Similarly, cell number and free auxin level were elevated in mutant plants while decreased in overexpression lines. Biochemical data indicated that CsRAX1/5 directly promoted the expression of auxin glucosyltransferase gene CsUGT74E2. Therefore, our data suggested that CsRAXs function as repressors for leaf size development by promoting auxin glycosylation to decrease free auxin level and cell division in cucumber. Our findings provide new gene targets for cucumber breeding with increased leaf size and crop yield.
650	1	4	_aCSRAXS
650	1	4	_aCSUGT74E2
650	1	4	_aAUXIN GLYCOSYLATION
650	1	4	_aFRUITING ABILITY
650	1	4	_aLEAF SIZE
700	1	2	_aChen, J.
700	1	2	_aLiu, L.
700	1	2	_aChen, G.
700	1	2	_aWang, S.
700	1	2	_aLiu, Y.
700	1	2	_aZhang, Z.
700	1	2	_aZhang, X
856	4	0	_uhttps://drive.google.com/open?id=1h9dDM0w-cMt5Zae1RBkxTpeVdBn-jYQq&usp=drive_copy _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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