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090			_aB-21188
245	1	0	_aNitric oxide acts downstream of reactive oxygen species in phytomelatonin receptor 1 (PMTR1)-mediated stomatal closure in Arabidopsis
490	0		_aJournal of Plant Physiology. 282, 153917, 2023, 10.1016/j.jplph.2023.153917
520	3		_aReactive oxygen species (ROS) and nitric oxide (NO) are important signaling molecules regulating stomatal movements in plants. Melatonin (N-acetyl-5-methoxytryptamine) was found to induce stomatal closure via phytomelatonin receptor 1 (PMTR1)-mediated activation of ROS production. Here, we evaluated the interaction between ROS and NO in the melatonin-induced stomatal closure in Arabidopsis. The results showed that the exogenous melatonin-induced stomatal closure and NO production were abolished by carboxy-PTIO (cPTIO, a NO scavenger). Additionally, the mutant lines nitrate reductase 1 and 2 (nia1nia2) and NO-associated 1 (noa1) did not show melatonin-induced stomatal closure, indicating that the melatonin-mediated stomatal closure is dependent on NO. The application of H2O2 induced the NO production and stomatal closure in the presence or absence of melatonin. However, the melatonin-induced NO production was impaired in the rhohC and rbohD/F (NADPH oxidase respiratory burst oxidase homologs) mutant plants. Furthermore, the ROS levels in nia1nia2 and noa1 did not differ significantly from the wild type plants, indicating that NO is a downstream component in the melatonin-induced ROS production. Exogenous melatonin did not induce NO and ROS production in the guard cells of pmtr1 mutant lines, suggesting NO occurs downstream of ROS in the PMTR1-mediated stomatal closure in Arabidopsis. Taken together, the results presented here suggest that melatonin-induced stomatal closure via PMTR1-mediated signaling in the regulation of ROS and NO production in Arabidopsis. © 2023 Elsevier GmbH
650	1	4	_aARABIDOPSIS THALIANA
650	1	4	_aMELATONIN
650	1	4	_aNITRIC OXIDE
650	1	4	_aPMTR1
650	1	4	_aROS
650	1	4	_aSTOMATAL CLOSURE
700	1	2	_aWang Z.
700	1	2	_aLi L.
700	1	2	_aKhan D.
700	1	2	_aChen Y.
700	1	2	_aPu X.
700	1	2	_aWang X.
700	1	2	_aGuan M.
700	1	2	_aRengel Z.
700	1	2	_aChen Q.
856	4	0	_uhttps://drive.google.com/file/d/1Ldm6pMSdx2JkVbjxeyc04jy-2L-gwnHm/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta @cicy.edu.mx
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