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090			_aB-21264
245	1	0	_aImmunostimulant effects of diet supplementation with yellow (Pouteria campechiana), white (Casimiroa edulis), and black (Diospyros digyna) sapote nanocapsules on laying hens: in vitro and in vivo study.
490	0		_aTropical Animal Health and Production. 55(6), 360-, 2023, DOI: https://doi.org/10.1007/s11250-023-03778-y
520	3		_aPoultry is commonly infected by different bacteria and parasites in the environment, resulting in increased morbidity and mortality, but immunostimulants have been enhancing non-specific defense mechanisms conferring laying hens' protection. For this purpose, the pulp of yellow (Pouteria campechiana), white (Casimiroa edulis), and black (Diospyros digyna) sapotes were nanoencapsulated (YWB-SN) and evaluated in laying hens' peripheral blood leukocytes to test their addition to the experimental diets at a concentration of 0.5% (5g/kg of dry food) for 1 month (with two samples at days 15 and 30). The YWB-SN were safe when exposed to peripheral blood leukocytes (PBLs). The in vitro experiment showed that these nanocapsules enhanced reactive oxygen species production, and B-SN stimulated phagocytosis activity. Concerning the proinflammatory cytokine (TNF-α) transcription, this gene was upregulated after W-SN stimulation, while B-SN upregulated the IgG gene expression significantly. IgM was upregulated with any YBW-SN in PBLs after 24 h of stimulation. The in vivo study showed a notable B-SN immunostimulation in serum and an upregulation of TNF-α, IgM, and IgG mRNA transcription. Therefore, this study provides a new result of the yellow, white, and black sapote nanocapsules as a functional food for the poultry industry, highlighting the black sapote Diospyros digyna immunostimulant effect.
650	1	4	_aPOUTERIA CAMPECHIANA
650	1	4	_aCASIMIROA EDULIS
650	1	4	_aDIOSPYROS DIGYNA
650	1	4	_aNANOCAPSULES
650	1	4	_aIMMUNE SYSTEM
650	1	4	_aLAYING HENS
700	1	2	_aCorona, L. R. D.
700	1	2	_aRodríguez, M. E. M.
700	1	2	_aPérez, L. M. A.
700	1	2	_aYerena, A. R.
700	1	2	_aMartínez Preciado, A. H.
700	1	2	_aReyes-Becerril, M.
856	4	0	_uhttps://drive.google.com/file/d/1iVULEwOHtZ2DqCYGXFDZh0IMk3G91otF/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta @cicy.edu.mx
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