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090			_aB-21314
245	1	0	_aCRISPR-Cas13d in plant biology: an insight.
490	0		_aPlant Biotechnology Reports. 18(3), 301-311, 2024, DOI: https://doi.org/10.1007/s11816-024-00893-6
520	3		_aPlants are among the many creatures that have benefited from the widespread application of the CRISPR-associated Cas system as a genome-editing tool for investigating gene function, identifying disease, and enhancing agricultural yields. Although the CRISPR/Cas systems for DNA editing are widely employed, post-transcriptional manipulation of RNA remains difficult despite the prevalence of Cas9. Type VI CRISPR/Cas systems, which were recently found, allow for precise RNA editing without permanently affecting the genome. Cas13d has been put to good use in RNA-related studies across a wide range of RNA knock-down, and RNA detection without affecting DNA. Regulation of cas13d specificity and activity helps to avoid the off-target effects and immune responses in plants. Cas13d as highly efficient RNA-targeting tools for the virus resistance, gene function studies, disease diagnostics, and crop improvement in plants. However, CRISPR/Cas13d applications in plant RNA biology are just getting started. This article discusses how RNA editing tools derived from the CRISPR/Cas13d system are currently being used and where they may be used in the future for plant research.
650	1	4	_aCRISPR
650	1	4	_aCAS13D
650	1	4	_aPLANT BIOLOGY
650	1	4	_aRNA EDITING
700	1	2	_aSarkar, J.
700	1	2	_aJyoti, T. P.
700	1	2	_aSahana, S.
700	1	2	_aBhattacharya, A.
700	1	2	_aChandel, S.
700	1	2	_aSingh, R.
856	4	0	_uhttps://drive.google.com/file/d/1KQuRqIJYcMdvsGew2mZ1gg8vrXQAnfg3/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta @cicy.edu.mx
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