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090			_aB-21374
245	1	0	_aSystematically amino acid analysis: Advancements in extinction coefficient determination for therapeutic proteins
490	0		_aAnalytical Biochemistry. 690, 115508, 2024, DOI: 10.1016/j.ab.2024.115508
520	3		_aBiologicals developers often face challenges in accurately determining the extinction coefficient (EC) measurement. We have successfully improved the precision and robustness of the widely recognized amino acid analysis method for EC determination, through a stepwise optimization process. Extensive analyses based on 114 observations, covering eight proteins over three years were performed, with a maximum relative standard deviation of 1.5% among multiple analysts, and a maximum deviation of 2.8% from the theoretical EC across the eight given proteins examined. © 2024 Elsevier Inc.
650	1	4	_aAMINO ACID ANALYSIS
650	1	4	_aEXTINCTION COEFFICIENT
650	1	4	_aPROTEIN QUANTITY
650	1	4	_aTHERAPEUTIC PROTEINS
700	1	2	_aXu Y.
700	1	2	_aShao Z.
700	1	2	_aZhang L.
700	1	2	_aCao Y.
700	1	2	_aLiu Z.
700	1	2	_aZhang Z.
856	4	0	_uhttps://drive.google.com/file/d/1DN9_ICCoE4uOxK7-0n9duOMAK8CGqMek/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta @cicy.edu.mx
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