| 000 | 02409nam a2200265Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625164354.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-21425 | ||
| 245 | 1 | 0 | _aProduction and immobilization of laccases from monoculture and co-culture of Trametes villosa and Pycnoporus sanguineus for sustainable biodegradation of ciprofloxacin |
| 490 | 0 | _aProcess Biochemistry. 141, 132-143, 2024, DOI: 10.1016/j.procbio.2024.03.012 | |
| 520 | 3 | _aThe presence of antibiotics in water systems and drinking water has detrimental effects on various organisms and poses health risks to both animals and humans. This study describes a sustainable approach using laccases to remove ciprofloxacin from water solutions. Laccases produced by monoculture of Trametes villosa and in co-culture with Pycnoporus sanguineus were immobilized using the method of cross-linked enzyme aggregates (CLEAs). The immobilization process was statistically optimized, and the biocatalysts were characterized and applied for biodegradation of ciprofloxacin at different concentrations. Laccase production was enhanced in co-culture conditions, yielding isoforms of molecular masses ranging from 55 to 45 kDa. Optimum conditions for immobilizing laccases by CLEAs were achieved with ammonium sulfate as a precipitant and glutaraldehyde as a cross-linker. Immobilization improved the thermal stability of the enzymes at 40 °C and 55 °C, and both forms of laccase CLEAs retained approximately 38% of their initial activity after five reuse cycles. Free and immobilized laccases demonstrated comparable efficiency in removing ciprofloxacin from aqueous solutions (at 2.5 mg L?1) with a 53-62% removal rate in the presence of 4-hydroxybenzoic acid as a natural redox mediator. The degradation of ciprofloxacin by laccases also reduced its antimicrobial activity against Escherichia coli. © 2024 Elsevier Ltd | |
| 650 | 1 | 4 | _aANTIBIOTICS |
| 650 | 1 | 4 | _aBIODEGRADATION |
| 650 | 1 | 4 | _aCROSS-LINKED ENZYME AGGREGATES |
| 650 | 1 | 4 | _aOXIDOREDUCTASE |
| 650 | 1 | 4 | _aWHITE-ROT FUNGI |
| 700 | 1 | 2 | _aBraga D.M. |
| 700 | 1 | 2 | _aBrugnari T. |
| 700 | 1 | 2 | _aHaminiuk C.W.I. |
| 700 | 1 | 2 | _aMaciel G.M. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1WK9POqWXv2G4Z8OLOVYcA1SIR48ixrDo/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta @cicy.edu.mx |
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