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245 1 0 _aA nitrogen-responsive cytokinin oxidase/dehydrogenase regulates root response to high ammonium in rice
490 0 _aNew Phytologist, https://doi.org/10.1111/nph.20128, 2024
500 _aArtículo
520 3 _aPlant root system is significantly influenced by high soil levels of ammonium nitrogen, leading to reduced root elongation and enhanced lateral root branching. In Arabidopsis, these processes have been reported to be mediated by phytohormones and their downstream signaling pathways, while the controlling mechanisms remain elusive in crops. Through a transcriptome analysis of roots subjected to high/low ammonium treatments, we identified a cytokinin oxidase/dehydrogenase encoding gene, CKX3, whose expression is induced by high ammonium. Knocking out CKX3 and its homologue CKX8 results in shorter seminal roots, fewer lateral roots, and reduced sensitivity to high ammonium. Endogenous cytokinin levels are elevated by high ammonium or in ckx3 mutants. Cytokinin application results in shorter seminal roots and fewer lateral roots in wild-type, mimicking the root responses of ckx3 mutants to high ammonium. Furthermore, CKX3 is transcriptionally activated by type-B RR25 and RR26, and ckx3 mutants have reduced auxin content and signaling in roots under low ammonium. This study identified RR25/26-CKX3-cytokinin as a signal module that mediates root responses to external ammonium by modulating of auxin signaling in the root meristem and lateral root primordium. This highlights the critical role of cytokinin metabolism in regulating rice root development in response to ammonium. © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
650 1 4 _aAMMONIUM
650 1 4 _aAUXIN
650 1 4 _aCKX
650 1 4 _aCYTOKININ
650 1 4 _aLATERAL ROOT
650 1 4 _aRICE
650 1 4 _aSEMINAL ROOT
700 1 2 _aLi, L.
700 1 2 _aJia, L.
700 1 2 _aDuan, X.
700 1 2 _aLv, Y.
700 1 2 _aYe, C.
700 1 2 _aDing, C.
700 1 2 _aXuan, W. A.
856 4 0 _uhttps://drive.google.com/file/d/1WUpx1-xgZ_7C7_ivDf_Y6Egd6zq9oKb-/view?usp=drive_link
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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