| 000 | 01113nam a2200217Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250626080925.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-15174 | ||
| 245 | 1 | 0 | _aThe Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells |
| 490 | 0 | _aCold Spring Harbor Protocol, 6, p.pdb-prot101196, 2020 | |
| 500 | _aArtículo | ||
| 520 | 3 | _aThis protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical. | |
| 650 | 1 | 4 | _aTRANSFORMATION |
| 650 | 1 | 4 | _aCOMPETENT ESCHERICHIA COLI |
| 700 | 1 | 2 | _aGreen, M. R. |
| 700 | 1 | 2 | _aSambrook, J. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1iANnzpPpF1gkFq9epIYnTLA1XNtna6zw/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 |
_2Loc _cREF1 |
||
| 008 | 250602s9999 xx |||||s2 |||| ||und|d | ||
| 999 |
_c55548 _d55548 |
||