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090			_aB-20378
245	1	0	_aCryopreservation of zygotic embryos derived from BBTV resistant accessions of wild banana Musa acuminata
490	0		_aSouth African Journal of Botany, 173, p.255-265, 2024
500			_aArtículo
520	3		_aThe wild banana species Musa acuminata encompasses numerous subspecies, some of which serve as ancestors of edible bananas. Preserving these subspecies is critical for food security, especially in regions where bananas are staple crops. While cryopreservation of M. acuminata subsp. burmanicoides zygotic embryos using air dehydration have shown high success rates, this method has not been effective for other subspecies. With at least 15 subspecies displaying various morphological traits, including seed morphology, alternative cryopreservation methods warrant exploration. In a recent study, successful cryopreservation using the droplet-vitrification method was achieved for mature zygotic embryos of accession of M. acuminata subspecies tomentosa, rutilifes, and malaccensis, all of which are resistant to the Banana bunchy top virus (BBTV). Using this approach, subjecting embryos to dehydration for 20 min in PVS2 solution and employing a regeneration medium comprising MS medium supplemented with benzyladenine and thidiazuron resulted in a success rate of 77.4 % (86 % survival rate × 90 % regrowth rate) for subsp. tomentosa and 50.4 % (70 % survival rate × 72 % regrowth rate) for subsp. malaccensis. Conversely, for subsp. rutilifes, dehydration for 10 min led to a success rate of 15.6 % (30 % survival rate × 52 % regrowth rate). Subspecies emerged as the primary factor influencing embryo regrowth, suggesting the need for protocol optimizations tailored to other subspecies. These findings highlight the potential of droplet-vitrification as a long-term preservation method for certain subspecies, while underscoring the necessity for further optimization for the other subspecies of M. acuminata.
650	1	4	_aMUSA ACUMINATA SUBSP. MALACCENSIS
650	1	4	_aSUBSP. RUTILIFES
650	1	4	_aSUBSP. TOMENTOSA
650	1	4	_aCONSERVATION
650	1	4	_aVITRIFICATION
650	1	4	_aDROPLET-VITRIFICATION
700	1	2	_aRoostika, I.
700	1	2	_aRahayu, E.M.D.
700	1	2	_aPrawestri, A.D.
700	1	2	_aPoerba, Y.S.
700	1	2	_aHandayani, T.
700	1	2	_aAhmad, F.
700	1	2	_aWidjaya, A.H.
700	1	2	_aMartanti, D.
700	1	2	_aHerlina
700	1	2	_aLatifah, D.
856	4	0	_uhttps://drive.google.com/file/d/1k0HHDYh2qD5uTb8_e4onSlZLpfH3qo6m/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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