| 000 | 02445nam a2200289Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250626083335.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-21612 | ||
| 245 | 1 | 0 | _aIdentification of StAP2/ERF genes of potato (Solanum tuberosum) and their multiple functions in detoxification and accumulation of cadmium in yest: Implication for Genetic-based phytoremediation |
| 490 | 0 | _aScience of The Total Environment, 810, p.152322, 2022 | |
| 500 | _aArtículo | ||
| 520 | 3 | _aThe discovery of genes responsible for the tolerance to heavy metals is critical for genome-based phytotechnologies. In this study, we exposed potato (Solanum tuberosum L.) to Cd/Pb/Zn/Ni/Cu as an approach to explore the potential genes associated with stress tolerance. Using genome-wide analysis, we identified 181 potential StAP2/ERF genes that were classified into three subgroups. These StAP2/ERF genes were significantly related to heavy metal stress and are more specifically related to Cd tolerance in yeast. Yeast complementation tests showed that the StAP2/ERF129/139 genes (Subgroup 1) decreased Cd accumulation (Cd reduction-type), whilst the StAP2/ERF044/180 genes (Subgroup 2) promoted Cd accumulation in yeast which showed inhibited growth (Cd accumulation-type). The StAP2/ERF075/077/ 126 genes (Subgroup 3) promoted Cd accumulation and yeast growth (Cd detoxification-type). We used phylogenetic analysis to classify the 181 genes into three Cd tolerant types defined above in which the numbers of Cd reduction, accumulation, and detoxification type genes were 81, 65 and 35 respectively. Also, we performed tandem duplication, phylogenetic, and conserved motifs analysis to characterization the StAP2/ERF genes and results supported their functions in Cd tolerance. Our study showed that StAP2/ERFs is indispensable in Cd uptake and tolerance, and may be useful towards designing gene-modified plants with improved Cd tolerances. | |
| 650 | 1 | 4 | _aHETEROLOGOUS EXPRESSION |
| 650 | 1 | 4 | _aPOTATO |
| 650 | 1 | 4 | _aAP2/ERF GENES |
| 650 | 1 | 4 | _aHEAVY METAL STRESS |
| 700 | 1 | 2 | _aTian, W. |
| 700 | 1 | 2 | _aHuang, Y. |
| 700 | 1 | 2 | _aLi, D. |
| 700 | 1 | 2 | _aMeng, L. |
| 700 | 1 | 2 | _aHe, T. |
| 700 | 1 | 2 | _aHe, G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1nQd1MIKg3SlUvOIpncCgRoUnuPkzJDKP/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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