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090			_aB-21623
245	1	0	_aInfluence of Nitrogen Balance of Culture Medium on Norway Spruce [Picea abies (L.) Karst] Somatic Polyembryogenesis: High Frequency Establishment of Embryonal-Suspensor Mass Lines from Mature Zygotic Embryos
490	0		_aJournal of Plant Physiology 142 (6):735-741, 1993
500			_aArtículo
520	3		_aProliferating embryonal-suspensor masses (ESMs) were initiated from mature open-pollinated seeds of a northern Norway spruce (Picea abies (L.) Karst) family possessed a relatively low level of embryogenic activity. Different nitrogen content modifications of the half-strength von Arnold and Eriksson (~ LP) basal medium were tested for both the induction of ESM formation and the establishment of ESM lines. Both organic and inorganic nitrogen strongly affected the ESM line establishment. Amide nitrogen (asparagine or glutamine) was an obligatory prerequisite for the ESM line establishment and prolonged maintenance. The optimal ammonium! nitrate molar ratio in the presence of 3.0 mM glutamine was about 0.2. The highest frequency ESM line establishment (33 percent) was obtained with 7.5 mM ammonium, 33.8 mM nitrate and 3.0 mM glutamine. Somatic embryo maturation was determined by the combined effects of the nitrogen balances of maintenance and maturation media. After a two-step maturation procedure, mature somatic embryos were successfully germinated and transferred ex vitrum.
650	1	4	_aPICEA ABIES
650	1	4	_aESTABLISHMENT OF EMBRYONAL-SUSPENSOR MASS LINES
650	1	4	_aNITROGEN SOURCES
650	1	4	_aNORWAY SPRUCE
650	1	4	_aSOMATIC POLYEMBRYOGENESIS
700	1	2	_aBozhkov, P. V.
700	1	2	_aMikhlina, S. B.
700	1	2	_aShiryaeva, G. A.
700	1	2	_aLebedenko, L. A.
856	4	0	_uhttps://drive.google.com/file/d/13f0gQ5e7H50JooorsW-9AEpelw0BTNwe/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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