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| 003 | MX-MdCICY | ||
| 005 | 20250626090915.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-21694 | ||
| 245 | 1 | 0 | _aAltering Endogenous Cytokinin Content by GmCKX13 as a Strategy to Develop DroughtTolerant Plants |
| 490 | 0 | _aPlant Stress, p.100678, 2024 | |
| 500 | _aArtÃculo | ||
| 520 | 3 | _aClimate-resilient crops are essential to meet the growing food demands of an ever-increasing world population. The phytohormone cytokinins (CKs) have been well established to regulate plant adaptation to drought. Previously, we reported that GmCKX13, encoding a CK oxidase/dehydrogenase in soybean (Glycince max), was differentially expressed under drought. Here, we further characterized its in planta function to assess if GmCKX13 could be a candidate gene to develop drought-tolerant crops. Transgenic Arabidopsis (35S:GmCKX13) plants ectopically and constitutively expressing GmCKX13 using the 35S promoter had an increase in root length, while showing a reduction in shoot height and biomass. CK contents were reduced in 35S:GmCKX13 plants, coupled with the reduced expression of all AtCKX genes and increased expression of all Arabidopsis isopentenyl transferases (AtIPTs), except AtIPT2 and AtIPT9 that are responsible for the production of cis-zeatin-type CKs. The 35S:GmCKX13 plants showed improved tolerance to drought and more sensitivity to the treatment with exogenous abscisic acid (ABA). Under the control of the drought-responsive promoter RD29A, the transgenic RD29A:GmCKX13 Arabidopsis plants had a similar phenotype as that of the wild-type (WT) plants under normal conditions. Under dehydration, we found a significant increase in the expression of the transgene coupled with a higher leaf relative water content in RD29A:GmCKX13 plants when compared with WT plants. In consistence with this finding, the RD29A:GmCKX13 plants exhibited higher survival rates and 30 percent higher seed yield than the WT plants under drought conditions. Taken together, our results demonstrated that GmCKX13 is an excellent gene for developing drought-tolerant crops by altering endogenous CK levels and ABA responsiveness when being driven by a drought-responsive promoter. | |
| 650 | 1 | 4 | _aCYTOKININ MODULATION |
| 650 | 1 | 4 | _aDROUGHT TOLERANCE |
| 650 | 1 | 4 | _aGMCKX13 |
| 650 | 1 | 4 | _aRD29A |
| 650 | 1 | 4 | _aTRANSGENIC |
| 700 | 1 | 2 | _aLe, D. T. |
| 700 | 1 | 2 | _aVan Ha, C. |
| 700 | 1 | 2 | _aNguyen, K. H. |
| 700 | 1 | 2 | _aChu, H. D. |
| 700 | 1 | 2 | _aZhu, C. |
| 700 | 1 | 2 | _aLi, W. |
| 700 | 1 | 2 | _aTran, L. S. P. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/13FIdaytlt5nvOgsX8hfS5CqgLx3nLUDS/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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