| 000 | 02392nam a2200229Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250626093125.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-21773 | ||
| 245 | 1 | 0 | _aTranscriptome analysis reveals molecular mechanisms underlying chloroplast biogenesis in albino Agave angustifolia plantlets |
| 490 | 0 | _aPhysiologia Plantarum, 176(2), p.e14289, 2024 | |
| 500 | _aArtículo | ||
| 520 | 3 | _aAlbino plants display partial or complete loss of photosynthetic pigments and defective thylakoid membrane development, consequently impairing plastid function and development. These distinctive attributes render albino plants excellent models for investigating chloroplast biogenesis. Despite their potential, limited exploration has been conducted regarding the molecular alterations underlying these phenotypes, extending beyond photosynthetic metabolism. In this study, we present a novel de novo transcriptome assembly of an albino somaclonal variant of Agave angustifolia Haw., which spontaneously emerged during the micropropagation of green plantlets. Additionally, RT‐qPCR analysis was employed to validate the expression of genes associated with chloroplast biogenesis, and plastome copy numbers were quantified. This research aims to gain insight into the molecular disruptions affecting chloroplast development and ascertain whether the expression of critical genes involved in plastid development and differentiation is compromised in albino tissues of A. angustifolia. Our transcriptomic findings suggest that albino Agave plastids exhibit high proliferation, activation of the protein import machinery, altered transcription directed by PEP and NEP, dysregulation of plastome expression genes, reduced expression of photosynthesis‐associated nuclear genes, disruption in the tetrapyrrole and carotenoid biosynthesis pathway, alterations in the plastid ribosome, and an increased number of plastome copies, among other alterations. | |
| 700 | 1 | 2 | _aAndrade‐Marcial, M. |
| 700 | 1 | 2 | _aPacheco‐Arjona, R. |
| 700 | 1 | 2 | _aHernández‐Castellano, S. |
| 700 | 1 | 2 | _aChe‐Aguilar, L. |
| 700 | 1 | 2 | _aDe‐la‐Peña, C. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1xW7oIPHozpKYWx3AIYFpMAN_95YmXQ5m/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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