000 02323nam a2200277Ia 4500
003 MX-MdCICY
005 20250626093125.0
040 _cCICY
090 _aB-21777
245 1 0 _aNovel solid-state fermentation of bee-collected pollen emulating the natural fermentation process of bee bread
490 0 _aFood Microbiology 82 (2019) 218-2
500 _aArtículo
520 3 _aStructure of lactic acid bacteria biota in ivy flowers, fresh bee-collected pollen (BCP), hive-stored bee bread, and honeybee gastrointestinal tract was investigated. Although a large microbial diversity characterized flowers and fresh BCP, most of lactic acid bacteria species disappeared throughout the bee bread maturation, giving way to Lactobacillus kunkeei and Fructobacillus fructosus to dominate long stored bee bread and honeybee crop. Adaptation of lactic acid bacteria was mainly related to species-specific, and, more in deep, to strain-specific features. Bee bread preservation seemed related to bacteria metabolites, produced especially by some L. kunkeei strains, which likely gave to lactic acid bacteria the capacity to outcompete other microbial groups. A protocol to ferment BCP was successfully set up, which included the mixed inoculum of selected L. kunkeei strains and Hanseniaspora uvarum AN8Y27B, almost emulating the spontaneous fermentation of bee bread. The strict relationship between lactic acid bacteria and yeasts during bee bread maturation was highlighted. The use of the selected starters increased the digestibility and bioavailability of nutrients and bioactive compounds naturally occurring in BCP. Our biotechnological protocol ensured a product microbiologically stable and safe. Conversely, raw BCP was more exposed to the uncontrolled growth of yeasts, moulds, and other bacterial groups.
650 1 4 _aBEE-COLLECTED POLLEN
650 1 4 _aFERMENTATION
650 1 4 _aBEE BREAD
650 1 4 _aLACTOBACILLUS KUNKEEI
650 1 4 _aHONEYBEE
700 1 2 _aDi Cagno, R.
700 1 2 _aFilannino, P.
700 1 2 _aCantatore, V.
700 1 2 _aGobbetti, M.
856 4 0 _uhttps://drive.google.com/file/d/1a17VdY1d-Eb9Ou5HjATE22-nJK2GBirE/view?usp=drive_link
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c55839
_d55839