000			03803nam a22004575i 4500
001			978-0-387-78175-4
003			DE-He213
005			20251006084418.0
007			cr nn 008mamaa
008			110409s2011 xxu| s |||| 0|eng d
020			_a9780387781754
020			_a99780387781754
024	7		_a10.1007/978-0-387-78175-4 _2doi
082	0	4	_a570.282 _223
100	1		_aPrice, Robert L. _eeditor.
245	1	0	_aBasic Confocal Microscopy _h[electronic resource] / _cedited by Robert L. Price, W. Gray (Jay) Jerome.
264		1	_aNew York, NY : _bSpringer New York, _c2011.
300			_aXI, 302p. 139 illus., 103 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aTable of Contents.- Chapter 1, Introduction and Historical Perspective.-Chapter 2, The Theory of Fluorescence.- Chapter 3, Fluorescence Microscopy.- Chapter 4, Specimen Preparation -- Chapter 5,  Labeling Considerations for Confocal Microscopy -- Chapter 6, Introduction to Digital Imaging for Confocal Microscopy -- Chapter 7, Digital Image Capture for Confocal Microscopy -- Chapter 8, Types of Confocal Instruments: Basic Principals and Advantages and Disadvantages.- Chapter 9, Setting the Operating Parameters.- Chapter 10, 3D Reconstruction of Confocal Image Data.- Chapter 11, Ethics and Resources.- Glossary  .
520			_aMost researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors' five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.
650		0	_aLIFE SCIENCES.
650		0	_aBIOTECHNOLOGY.
650		0	_aCYTOLOGY.
650		0	_aMICROSCOPY.
650	1	4	_aLIFE SCIENCES.
650	2	4	_aBIOLOGICAL MICROSCOPY.
650	2	4	_aCELL BIOLOGY.
650	2	4	_aBIOTECHNOLOGY.
700	1		_aJerome, W. Gray (Jay). _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9780387781747
856	4	0	_uhttp://dx.doi.org/10.1007/978-0-387-78175-4 _zVer el texto completo en las instalaciones del CICY
912			_aZDB-2-SBL
942			_2ddc _cER
999			_c59051 _d59051