000			03142nam a22003855i 4500
001			978-1-4020-3319-3
003			DE-He213
005			20251006084454.0
007			cr nn 008mamaa
008			100301s2005 ne | s |||| 0|eng d
020			_a9781402033193
020			_a99781402033193
024	7		_a10.1007/1-4020-3319-2 _2doi
082	0	4	_a610 _223
100	1		_aBienvenut, Willy Vincent. _eeditor.
245	1	0	_aAcceleration and Improvement of Protein Identification by Mass Spectrometry _h[electronic resource] / _cedited by Willy Vincent Bienvenut.
264		1	_aDordrecht : _bSpringer Netherlands, _c2005.
300			_aXXII, 298 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aPreface -- Acknowledgements -- List of Contributors -- 1: Introduction: Proteins analysis using mass spectrometry -- 2: Molecular scanner development: Toward clinical molecular scanner for proteome research: Parallel protein chemical processing before and during western-blot -- 3: Quantitation during electroblotting step: Enhanced protein recovery after electrotransfer using square wave alternating voltage -- 4: Signal traitment and virtual imaging (1/2): A molecular scanner to highly automated research and to display proteome images -- 5: Signal traitment and virtual imaging (2/2): Visualization and analysis of molecular scanner peptide mass spectra -- 6: Improvement in the peptide mass fingerprint protein identification (1/2): Hydrogen/deuterium exchange for higher specificity of protein identification by peptide mass fingerprinting -- 7: Improvement in the peptide mass fingerprint protein identification (2/2): MALDI-MS/MS with high resolution and sensitivity for identification and characterization of proteins -- 8: Proteomic and mass spectrometry: Some aspects and recent developments -- 9: Conclusions and perspectives -- Appendix -- Abbreviations used in this book -- Abbreviations for usual amino acids and chemical constants -- Index.
520			_aAt present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'. Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.
650		0	_aMEDICINE.
650	1	4	_aBIOMEDICINE.
650	2	4	_aBIOMEDICINE GENERAL.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781402033186
856	4	0	_uhttp://dx.doi.org/10.1007/1-4020-3319-2 _zVer el texto completo en las instalaciones del CICY
912			_aZDB-2-SBL
942			_2ddc _cER
999			_c60412 _d60412