| 000 | 02655nam a22004095i 4500 | ||
|---|---|---|---|
| 001 | 978-1-4020-3404-6 | ||
| 003 | DE-He213 | ||
| 005 | 20251006084455.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s2005 ne | s |||| 0|eng d | ||
| 020 | _a9781402034046 | ||
| 020 | _a99781402034046 | ||
| 024 | 7 |
_a10.1007/1-4020-3404-0 _2doi |
|
| 082 | 0 | 4 |
_a610 _223 |
| 100 | 1 |
_aViljoen, Gerrit J. _eauthor. |
|
| 245 | 1 | 0 |
_aMolecular Diagnostic PCR Handbook _h[electronic resource] / _cby Gerrit J. Viljoen, Louis H. Nel, John R. Crowther. |
| 264 | 1 |
_aDordrecht : _bSpringer Netherlands, _c2005. |
|
| 300 |
_aXVIII, 307 p. _bonline resource. |
||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 347 |
_atext file _bPDF _2rda |
||
| 505 | 0 | _aBackground -- PCR - The Basic Reaction -- Amplicon Detection, Analysis and PCR Evaluation -- Limitations and Troubleshooting -- Specific Types of PCR Assays -- The PCR Laboratory -- Disease Diagnosis Using PCR Specific Procedures for Important Veterinary Pathogens. | |
| 520 | _aThis book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology. | ||
| 650 | 0 | _aMEDICINE. | |
| 650 | 1 | 4 | _aBIOMEDICINE. |
| 650 | 2 | 4 | _aBIOMEDICINE GENERAL. |
| 700 | 1 |
_aNel, Louis H. _eauthor. |
|
| 700 | 1 |
_aCrowther, John R. _eauthor. |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer eBooks | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781402034039 |
| 856 | 4 | 0 |
_uhttp://dx.doi.org/10.1007/1-4020-3404-0 _zVer el texto completo en las instalaciones del CICY |
| 912 | _aZDB-2-SBL | ||
| 942 |
_2ddc _cER |
||
| 999 |
_c60444 _d60444 |
||