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245 1 0 _aBiomolecule repository of endophytic bacteria from guava serves as a key player in suppressing root- knot nematode, Meloidogyne enterolobii
490 0 _aScientia Horticulturae, 324, p.112627, 2024
500 _aArtículo
520 3 _aGuava production in India is hampered by invasion of guava root knot nematode, Meloidogyne enterolobii. Biological control of plant parasitic nematodes is the best strategy to quench the soil and root population of nematodes. Hence the attempts were made to dissect the endophytic bacteria from various niche of guava plants. Amongst the various endophytic bacteria’s, Klebsiella quasivaricola inhibited the egg hatching (100percent) and moratlity of juveniles (100percent). Exploration of non-volatile organic compounds of K. quasivaricola exhibited the presence of 2″-terthiophene, 9′spirobifluorenone, 4-Cyanophenyl 4-butylbenzoate, Benzoic acid, Benzoyl chloride, 3-phenyl-1, 2, 4-triazolin-5-one, Ethanone, 2,3-dihydro-5‑methoxy-, Ethanone, Trifluoroacetophenone, 6,12-dinaphthylindolo[3,2-b] carbazole having anti-microbial activities. Considering this, molecular docking experiments were carried out with important proteins of M. enterolobii such as Translationally Controlled Tumour Proteins, pectate lyase, phospholipase, hexokinase and Sphingomyelinase to understand the effect of biomolecules from K. quasivaricola. The results indicated that all biomolecules had prominent effect on different protein targets and in specific binding affinity with amino acid residues of protein targets benzoic acid, 4-Cyanophenyl 4-butylbenzoate, Trifluoroacetophenone, 6,12-dinaphthylindolo[3,2-b]carbazole, 3-phenyl-1,2,4-triazolin-5-one had significant effect on aforementioned protein targets of M. enterolobii. In addition, biohardened guava plants with the effective bacterial endophytes were challenged with M. enterolobii showcased 80percent increase on plant growth parameters and 70percent reduction of egg mass over control. Hence the present study gave limelight to explore the novel biomolecules from endophytic bacterium which could serve as an alternative synthetic nematicides for the management of M. enterolobii.
650 1 4 _aPLANT PARASITIC NEMATODES
650 1 4 _aBIOLOGICAL CONTROL
650 1 4 _aNEMATICIDAL ACTIVITY
650 1 4 _aMODE OF ACTION
650 1 4 _aMOLECULAR MODELLING AND DOCKING
700 1 2 _aGaneshan, S.; Annaiyan, S.; Somasundaram, P.; Mannu, J.; Kathithachalam, A.; Shanmugam, H.; Arunachalam, A.
856 4 0 _uhttps://drive.google.com/file/d/1LHoiAQa5RazA1jMF43XNmNT_oUGMZtXv/view?usp=drive_link
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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