000 02243nam a2200253Ia 4500
003 MX-MdCICY
005 20251009160706.0
040 _cCICY
090 _aB-21808
245 1 0 _aMorphological kinetics and distribution in somatic embryo cultures
490 0 _aBiotechnology and Bioengineering, 44, p.368-378, 1994
500 _aArtículo
520 3 _aThe environmental effects on developing somatic embryos should be characterized not only by the growth based on biomass, but also by the morphological properties and size. We have previously developed a discrete classifier to separate developing embryos into distinct morphological classes. In this study, a continuous descriptor using the distributions of magnitude of features representing morphological characteristics and size information was used to describe the developing embryo populations. The identity of the population was examined by comparing either the distributions of all features or of key features. The method was applied to characterize the kinetics of carrot embryo populations cultivated in the presence and absence of triiodobenzoic acid (TIBA), an inhibitor of auxin polar transport. Optimal sample size for morphological characterization was determined by the invariance of feature distributions with further increase in sample size. The overall growth and substrate consumption kinetics were only slightly affected by the presence of TIBA. However, the distribution of morphological features was significantly affected. The features showing the highest statistical significance were related to those corresponding to the roughness. The continuous descriptor for characterizing developing embryo population is potentially useful for quality control in large-scale operations.
650 1 4 _aDAUCUS CAROTA L.
650 1 4 _aEMBRYOS
650 1 4 _aKINETICS
650 1 4 _aMORPHOLOGY
650 1 4 _aPATTERN RECOGNITION
650 1 4 _aIMAGE ANALYSIS
700 1 2 _aChi, C. M.;Vits, H.;Staba, E. J.;Cooke, T. J.;Hu, W. S.
856 4 0 _uhttps://drive.google.com/file/d/1tDb2iiAZCV_aJazRUUfGPpiBp219Cu-a/view?usp=drive_link
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 251009s9999 xx 000 0 und d
999 _c61899
_d61899