000			02776nam a2200313Ia 4500
003			MX-MdCICY
005			20251009160707.0
040			_cCICY
090			_aB-21863
245	1	0	_aSNORD60 promotes the tumorigenesis and progression of endometrial cancer through binding PIK3CA and regulating PI3K/AKT/mTOR signaling pathway.
490	0		_aMolecular Carcinogenesis 62(4), 413-426, 2023
500			_aArtículo
520	3		_aEndometrial carcinoma is a common gynecological malignant tumor, small nucleolar RNAs (snoRNAs) are involved in cancer development. However, researches on the roles of snoRNAs in endometrial carcinoma are limited. The expression levels of snoRNAs in endometrial cancer tissues were analyzed using The Cancer Genome Atlas (TCGA) database. Antisense oligonucleotides (ASOs) and plasmids were used for transfection. Moreover, CCK‐8, EdU, wound‐healing assay, transwell, cell apoptosis, western blotting, and xenograft model were employed to examine the biological functions of related molecules. real‐time reverse transcription polymerase chain reaction and western blotting were performed to detect messenger RNA (mRNA) and protein levels. Including bioinformatics, fluorescence in situ hybridization, RNA pulldown, actinomycin D and RTL‐P assays were also carried out to explore the molecular mechanism. Analysis of data from TCGA showed that the expression level of small nucleolar RNA, C/D box 60 (SNORD60) in endometrial cancer tissues is observably higher than that in normal endometrial tissues. Further research suggested that SNORD60 played a carcinogenic role both in vitro and in vivo, and significantly upregulated the expression of PIK3CA. However, the carcinogenic effects can be reversed by knocking down fibrillarin (FBL) or PIK3CA. SNORD60 forms complexes by binding with 2′‐O‐methyltransferase fibrillarin, thus catalyzes the 2′‐O‐methylation (Nm) modification of PIK3CA mRNA and modulates the PI3K/AKT/mTOR signaling pathway, so as to promote the development of endometrial cancer. In short, SNORD60 might become a new biomarker for the therapy of endometrial cancer in the future and provide new strategies for diagnosis and treatment.
650	1	4	_a2′‐O‐METHYLATION
650	1	4	_aENDOMETRIAL CARCINOMA
650	1	4	_aNONCODING RNA
650	1	4	_aPIK3CA
650	1	4	_aSNORNAS
700	1	2	_aWu, W.
700	1	2	_aChen, X.
700	1	2	_aLiu, X.
700	1	2	_aBao, H. J.
700	1	2	_aLi, Q. H.
700	1	2	_aXian, J. Y.
700	1	2	_aChen, S.
856	4	0	_uhttps://drive.google.com/file/d/1SeLyeCt68HLtUJQKIOC0RWYb_7IAAMbA/view?usp=drive_link _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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