000			02982nam a2200313Ia 4500
001			000008275
003			MX-MdCICY
005			20260112162205.0
008			130617s2014 nyua f p 0|1 | eng d
020			_a9781621820789 (cloth)
020			_a9781936113583 (pbk)
040			_cCICY
082	0	4	_aC 572.516 _bC35 2014
245	1	0	_aCalcium techniques : _ba laboratory manual / _c[edited by] Jan B. Parys, Martin D. Bootman, David I. Yule, Geert Bultynck
264	3	1	_aNew York : _bCold Spring Harbor Laboratory Press, _cc2014
300			_axvi, 592 p. : _bil. ; _c28 cm.
504			_aIncluye referencias bibliográficas e índice
520	3		_aLife begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to ra úsele contraction and nerve impulses. To créate spatiotemporally distinct Ca2+ signáis, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principies and applica-tions of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imag-ing modalities to characterize Ca2+ signáis. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and meth-ods to investígate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and inter-cellular signáis are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in nonmammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques
650	1	4	_aCALCIO, CANALES _vMANUALES DE LABORATORIO
650	1	4	_aCALCIO, SEÑALES _vMANUALES DE LABORATORIO
650	1	4	_aCALCIO _vMANUALES DE LABORATORIO _xFISIOLOGIA
650	1	4	_aFLUOROMETRIA _vMANUALES DE LABORATORIO _xMETODOS
700	1	2	_aBootman, Martin D., _eed.
700	1	2	_aBultynck, Geert, _eed.
700	1	2	_aParys, Jan B., _d1958-, _eed.
700	1	2	_aYule, David I., _d1964-, _eed.
856	4	0	_uhttps://www.cicy.mx/sitios/sib/doctoelectronico/8275.pdf _zVer tabla de contenido y/o resumen
942			_2ddc _cBK
999			_c7568 _d7568